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Low temperature oxidation of linseed oil: a review
Fire Science Reviews volume 1, Article number: 3 (2012)
This review analyses and summarises the previous investigations on the oxidation of linseed oil and the self-heating of cotton and other materials impregnated with the oil. It discusses the composition and chemical structure of linseed oil, including its drying properties. The review describes several experimental methods used to test the propensity of the oil to induce spontaneous heating and ignition of lignocellulosic materials soaked with the oil. It covers the thermal ignition of the lignocellulosic substrates impregnated with the oil and it critically evaluates the analytical methods applied to investigate the oxidation reactions of linseed oil.
Initiation of radical chains by singlet oxygen (1Δg), and their propagation underpin the mechanism of oxidation of linseed oil, leading to the self-heating and formation of volatile organic species and higher molecular weight compounds. The review also discusses the role of metal complexes of cobalt, iron and manganese in catalysing the oxidative drying of linseed oil, summarising some kinetic parameters such as the rate constants of the peroxidation reactions.
With respect to fire safety, the classical theory of self-ignition does not account for radical and catalytic reactions and appears to offer limited insights into the autoignition of lignocellulosic materials soaked with linseed oil. New theoretical and numerical treatments of oxidation of such materials need to be developed. The self-ignition induced by linseed oil is predicated on the presence of both a metal catalyst and a lignocellulosic substrate, and the absence of any prior thermal treatment of the oil, which destroys both peroxy radicals and singlet O2 sensitisers. An overview of peroxyl chemistry included in the article will be useful to those working in areas of fire science, paint drying, indoor air quality, biofuels and lipid oxidation.
Since the 15th century, linseed oil has been extensively used in varnishes and oil-based house paints (Lazzari & Chiantore1999). It has also been applied for treating wood and in manufacturing of linoleum, a floor covering made from mixture of natural materials, such as wood, calcium, vegetable pigments and resin. Nowadays, it is also utilised in industrial lubricants, for the treatment of leather products, for bicycle maintenance, as well as rust inhibitor. Many studies have focussed on improving the drying performance of this oil and reducing the hazardous properties related to its application.
A herbaceous plant, Linum usitatissimum, linseed (also called flax) produces seeds which are oval and flattened in shape, 4–6 cm long, pale to dark brown and shiny. The oil prepared by crushing the seeds finds applications in formulating the so-called drying alkyd paints, which exhibit drying and hardening properties when exposed to air. This occurs as a consequence of high content of glycerol esters (also known as glycerides or triacylglycerols) of linolenic acid in linseed oil, an important component of the drying alkyd paints, with the unsaturated bonds in the acids undergoing the oxidation reactions. The non-drying alkyd resins are devoid of esters of unsaturated fatty acid and display no curing behaviour.
The properties of drying alkyd paints vary depending on the type and amount of unsaturated fatty acid employed in preparing the paint formulations (Oyman et al.2005a). The primary remaining components of both the drying and non-drying alkyd resins include al cohols (polyols), such as pentaerythritol or glycerol, and dicarboxylic acid anhydrides, such as phthalic and maleic anhydrides. The term alkyd derives from the original acronym alcid, conveying the chemical meaning of polyesters (van Gorkum & Bouwman2005).
There are four varieties of linseed oil sold in the market, including raw, boiled, stand and refined linseed oils. Raw linseed oil refers to pure oil with no additional treatment and with no additives, while boiled linseed oil is produced by adding a mixture of hydrocarbon solvents and metallic dryers to speed its drying time. Boiled oil is a well known trade name, even though the process does not involve boiling of raw oil, whereas the stand linseed oil is processed by heating the oil to about 300°C, over a few days in the absence of oxygen. During this process, polymerisation reaction occurs, increasing the oil’s viscosity. It means that stand or polymerised oil has been boiled to make it unreactive and more viscous. The production of refined linseed oil involves the alkali treatment following the pressing process, improving the colour of this oil (paler and clearer). It is utilised as a medium to increase gloss and transparency of paint colours.
The drying rate of linseed oil is too slow for convenient applications, necessitating the addition of metallic salts (drying agents) to accelerate the drying process (Mallégol et al.2000). Unfortunately, in the presence of lignocellulosic fuels, such as cotton fibres, these agents may induce the fuel’s self-heating and autoignition. This dangerous side effect of the oil has been known to fire investigators for almost 200 years (Abraham1996). Several cases of fire have been reported; in particular those induced by improperly disposed rags soaked with linseed oil. Typical ignition scenarios involve waste baskets filled with disposed cotton rags used to clean paint brushes.
Two recent cases of fire ignited by oily rags in California and Illinois, have been reported, one case occurred in the plant section where wooden cabinets have just been stained and finished, while the other was caused by a pile of oily rags in the storage area which had been used to treat the refinish deck. There were no injuries in either case, however they suffered estimated loss of $200,000 and $2,000, respectively. This substantial difference in losses resulted from the operation of sprinklers, in the case of the fire in Illinois (Evarts2011).
The US Fire Administration’s National Fire Incident Reporting System (NFIRS) and National Fire Protection Association (NFPA), which provide the average annual data of fires for 2005–2009, give account that the spontaneous heating of oily rags comprised 22% of fires ignited by spontaneous heating or chemical reaction. In 14,070 cases of fires caused by spontaneous heating or chemical reaction, there were 7 civilian deaths and 92 civilian injuries reported, with corresponding direct property damage of $143 millions (Evarts2011).
This review summarises the previous research studies dealing with oxidation of linseed oil itself and spon taneous ignition of lignocellulosic materials wetted with linseed oil. The review commences with the discussion of linseed oil composition, structure and oxidation characteristics. Several test methods for examining the oxidation and self-heating processes are then described, with application to the considered material. This is followed by illustrations of the effect of several transitional metals on the oxidation process. Subsequently, we discuss the chemistry involved in the oxidation reaction and the reaction pathways suggested in the literature, as well as the reported kinetic parameters. Finally, this review identifies the gaps in knowledge and proposes further investigation to gain improved fundamental understanding of the oxidation of linseed oil.
Characteristics of linseed oil
Composition and structure of linseed oil
As linseed is grown in several geographical areas, including Europe, North and South America (especially Argentina) and Asia (especially India), the linseed oil pressed from the seeds displays natural variation in composition that reflects the growing, agronomic and environmental conditions (Gunstone1996). In particular, climate affects the abundance of the unsaturated fatty acid in the oil; the colder the climate, the higher the iodine value of oil or the degree of its unsaturation (Fjällström et al.2002). Iodine value is normally expressed in terms of grams of iodine added per 100 grams of oil. The oil consists almost exclusively of the esters of glycerol (C3 alcohol with three hydroxyl groups, one on each carbon atom) and five fatty acids, two of them saturated, C16 palmitic and C18 stearic, and three unsaturated, oleic, linoleic and linolenic, exhibiting one, two and three double bonds, respectively, as illustrated in Figure 1. The two and three double bonds are non-conjugated, being separated from each other by CH2 groups. The minor components that may also be present are monoacylglycerols (monoesters of glycerol), diacylglycerols (diesters of glycerol) and free fatty acids (Gunstone1996).
Although linseed oil contains around 60% of linolenic acid, the most unsaturated fatty acid, this acid occurs only in small amounts, usually below 1% in other oil types. The notable exceptions are soybean and rape oil, with 8 and 7% linolenic content, respectively (Gunstone1996). This high level of linolenic acid in linseed oil affects the drying property of the oil, making it particularly suitable in formulations of drying alkyd paints. Table 1 summarises the composition of various natural oil; the symbols 16:0, 18:0, 18:1, 18:2 and 18:3 reflect the number of carbon atoms and (after the colon) the number of double bonds. They correspond to palmitic, stearic, oleic, linoleic and linolenic acids, respectively.
The natural unsaturated fatty acids exhibit one or more carbon double bonds with cis configuration starting at the location of the ninth carbon atom (Roberfroid & Calderon1995). Carbon-carbon double bonds in unsaturated fatty acids induce the chain to deviate by an angle of about 40°, requiring more space to accommodate the chain conformation (Jennings1981). The chemical structures of oleic, linoleic and linolenic acid are illustrated below Scheme 1:
Oleic acid incorporates 18 carbon atoms with one double bond at the position of the ninth carbon atom (counting from the carboxyl end) and displays a cis configuration. The index α refers to the numbering system, starting from the carboxyl end, while ω corresponds to that from the end of the carbon chain. Note that, each angle in the structures corresponds to the location of a carbon atom. Also, note that by organic chemistry convention, hydrogen atoms connected to carbon atoms are not shown. That is, although not shown, each carbon atom is bonded with hydrogen atoms, to make the total number of bonds for each carbon to be four.
Linoleic acid has the same length as oleic acid, but its structure includes two double bonds at positions 9 and 12 (counting from the location of the carboxyl group (Scheme 2)). Each double bond forms a cis configuration with the adjacent methylene (CH2) groups.
The chain of 18 carbon atoms with three double bonds at carbon atoms 9, 12 and 15 defines a molecule of linolenic acid (Scheme 3). Similarly to the other two naturally occurring C18 fatty acids, the double bonds in linolenic acid entail triple cis configuration. As the location of the first double bond is at the third carbon, counting from the end of the molecule, linolenic acid is an example of the so-called ω-3 fatty acids, one of the essential unsaturated fatty acids with well known health benefits. For this reason, linseed oil serves as nutritional supplement. The same nomenclature denotes linoleic and oleic acids as ω-6 and ω-9 fatty acids, respectively, with linoleic acid providing similar essential health benefits, especially as a precursor to arachidonic acid (20:4, ω-6), which controls several physiological functions in the human body. It is required for muscle growth and brain protection from oxidative stress.
Furfural (furan-2-carbaldehyde) serves as the preferred solvent for extraction of vegetable oils due to its miscibility and the ability of leaching out different types of oils as characterised by spread of iodine values. The tendency of furfural to form dark residues, accelerated by its exposure to heat, light and oxygen, constitutes its main disadvantage. In particular, these residues inhibit the oxidation of oil, retarding the formation of the paint film (Kenyon et al.1948).
Linseed oil has been reported to contain 15.2 meq/kg of hydroperoxides, determined using iodometric method (Peinado et al.1986); meq denotes mmol of monohydroperoxide equivalent to all hydroperoxide species. These peroxides probably build up during the extraction process. They may be generated from chemical oxidation involving heat or by the action of lipoxygenase enzyme, also called LOXes (linoleate:oxygen oxidoreductase). The enzyme belongs to a large family of non-iron containing fatty acid dioxygenases (Liavonchanka & Feussner2006). These enzymes occur widely in plants (cucumber, soybean, potato, sunflower) and animals (mammals) (Gunstone1996; Liavonchanka & Feussner2006). The degradation process of lipid bodies in the plant seeds during early stages of germination results in the formation of several enzymes such as 13-lipoxygenase, phospholipase and triacylglycerol lipase (Feussner et al.2001). This means that enzymes are present naturally in flax seeds. In particular, the lipoxygenase enzyme catalyses the oxidation reaction of polyunsaturated fatty acids with cis,cis-1,4-dienes structure to form hydroperoxides (Gunstone1996).
Oxidation process of linseed oil
In biology, unsaturated fatty acids function as important biomolecules in cellular metabolism (Roberfroid & Calderon1995). The drying of alkyd paint follows a mechanism similar to the process in which lipids are oxidised in biological systems (Miccichè et al.2006). Lipid peroxidation reactions occur in living systems, for instance in modification of DNA and proteins, radiation damage, aging and age pigment formation, modification of membrane structure, tumor initiation, and in the deposition of arterial plaque associated with low-density lipoprotein modification (Porter et al.1995). While in food industry, the important parameter to measure the quality of oil and fat is the degree of oxidation (i.e., the extent of oil oxidation), since this process reduces the nutritional quality, produces rancid flavours and decreases safety in terms of its potential to develop disease (Muik et al.2005).
Drying of alkyd paints consists of physical and chemical stages, the latter denoted as curing. In the first (physical) stage, the solvent evaporates prompting the formation of closed film, while the chemical drying involves lipid autoxidation, during which the cross-linking occurs (van Gorkum & Bouwman2005; Erich et al.2006a; Ploeger et al.2009a). This drying process is a consequence of the presence of linseed oil in the paints.
Linseed oil has the capacity to form a continuous film with good optical and mechanical properties after being spread out in a thin layer (Lazzari & Chiantore1999). It will cure in air without using a catalyst, although slowly (Marshall1986). The double bonds of the unsaturated acids in the oil react with oxygen in air and with one another to form a polymeric network (Lazzari & Chiantore1999; Stava et al.2007) that determines the drying power of such oils (Lazzari & Chiantore1999), resulting in the liquid layer evolving to a solid film (Roberfroid & Calderon1995; Marshall1986). Thus, this hardening process arises as a result of the autoxidation followed by cross-linking polymerisation (Lazzari & Chiantore1999; Fjällström et al.2002; Tanase et al.2004). The formation of cross linked structures essentially consists of the intermolecular coupling of radicals originated by decomposition of the relatively unstable peroxide groups (Lazzari & Chiantore1999). The drying reaction of linseed oil continues for many years even when the oil film seems to completely dry in a few days (Lazzari & Chiantore1999). However, the presence of glycerides as plasticisers can moderate the hardening process (Lazzari & Chiantore1999). The oil can gain up to 40% of its original weight during oxidation in the drying process with some weight loss due to the decomposition and disappearance of volatile compounds during oxidation reaction (Fjällström et al.2002).
Metals, light, heat and enzymes accelerate the oxidation of unsaturated acids in the oil, while antioxidants inhibit oxidation. Oxidation of fatty acids occurs through autoxidation or photo-oxidation. The rate of autoxidation depends on the presence of pro-oxidants (i.e., oxidising agents), antioxidants, temperature and the dissolved oxygen (Belhaj et al.2010). Oleic acid, as a monounsaturated acid, can be oxidised only at elevated temperatures, while polyunsaturated acids such as linolenic and linoleic acids undergo rapid oxidation even at room temperature (Kumarathasan et al.1992). Allyl hydroperoxides, chemical moieties containing both allyl (−CH = CH–CH2–) and hydroperoxide (−OOH) groups as in –CH = CH–CH(OOH)–, constitute the primary oxidation products. The double bonds remain but may have changed position and/or configuration from their original form during the oxidation reaction, with the formation of hydroperoxides. The schematic diagram in Figure 2 illustrates further changes affecting the hydroperoxides (Gunstone1996).
Autoxidation is a chain reaction process, with radical intermediates (odd electron species) of polyunsaturated fatty acids involved in initiation, propagation and termination steps (van Gorkum & Bouwman2005; Gunstone1996; Kumarathasan et al.1992). A slow induction period often precedes a more rapid reaction (Gunstone1996). On the other hand, photo-oxygenation reactions (oxidation reactions that are induced by light) involve attacks of singlet (i.e., excited) oxygen molecules, formed from triplet (i.e., ground state) oxygen by light in the presence of a sensitiser. This type of reaction shows no induction period and no inhibition by antioxidants. Table 2 lists the rates of photo-oxygenation reactions that significantly exceed those of the autoxidation reactions, particularly for monoene esters; note 30000 times enhancement (Gunstone1996). Lazzari observed that thermal treatment at 80°C can give an acceleration of around 40 times and photo ageing of approximately 260 times compared with natural ageing (Lazzari & Chiantore1999).
A characteristic pungent smell arising from air drying of alkyd coating originates from the formation of volatile by-products, especially aldehydes (Oyman et al.2007). In addition to the saturated aldehydes, small amounts of unsaturated aldehydes are also emitted (Fjällström et al.2002), as are short-chain ketones, alcohols, acids, esters, ethers and hydrocarbons, all contributing to the odour (Gunstone1996). Temperature and humidity affect the total emission of aldehydes, with the increasing temperature promoting the emissions and increasing humidity impeding them (Fjällström et al.2002). Cyclic fatty acid monomers (CFAM) also emerge during heating of linseed oil, with CFAM-18:2 consisting of mostly C5-membered-rings and CFAM-18:3 containing a mixture of C5 and C6 membered-rings (Joffre et al.2001). Figure 3 shows the example of CFAM.
Experimental methods to test oxidation and self-heating reactions of linseed oil
Several test methods exist to assess the propensity of materials to self-heating. Those that have been applied to cotton, sawdust and similar materials impregnated with linseed include basket heating, crossing point temperature, adiabatic reactor, differential thermal analysis (DTA), thermogravimetric analysis (TGA), Ordway apparatus and Mackey apparatus, as described in the following discussion.
Basket heating apparatus consists of a container (basket) shaped as a cube, a cylinder or a sphere, made of wire mesh to hold the sample, an oven to maintain the temperature and a thermocouple to measure the temperature inside the sample. Measuring the runaway temperature involves the following steps: charging the basket with a sample, inserting the thermocouple in the middle of the basket, then placing this basket inside a preheated oven and finally recording the temperature with a data logger (Wang et al.2006). The objective of this method is to determine the critical oven temperature that gives rise to self-heating. This approach assumes a single Arrhenius reaction rate, constant and isotropic material properties, and no water evaporation (Wang et al.2006; Drysdale1985). The measurements are normally analysed using the Frank-Kamenetskii theory as explained later in this review. Bowes et al. implemented this method to measure the self-heating and ignition temperature of sawdust for different concentrations of oxygen (Bowes & Thomas1966). This method is time and material consuming since a great number of tests must be performed to obtain the critical temperature. Moreover, locating the critical temperature is not straightforward. Worden attempted to implement this method to study the spontaneous ignition of cotton soaked with linseed oil, however, the critical temperature could not be determined due to difficulties in ascertaining the sub-critical conditions (Worden2011). In other words, using boiled oil (oil with cobalt accelerant), Worden could not find conditions that did not ignite. This leads one to conclude that there is no safe quantity of rags soaked with boiled linseed oil, and that even a single oil soaked rag at room temperature can self-heat. In practical terms, these findings question the classical theory of self-heating that stipulates the existence of a sub-critical condition for which a material does not self-ignite. The classical theory of self-heating does not cover self-ignition reaction involving radical and catalytic reactions. New theoretical and numerical treatments remain to be developed for spontaneous ignition induced by radical and catalytic reactions.
Crossing point methods of Chen and Jones
The purpose of both crossing point methods and that of adiabatic reactor, to be discussed in the next section, is to derive the kinetic parameters, E and A, which can then be applied to estimate the critical size of a material. The crossing point method of Chen, incorporates the following procedure: packing the solid particles into a steel mesh basket that has a steel mesh cover, followed by placing two thermocouples into the sample, one positioned at the centreline and the other at a small distance away, then situating the basket in the preheated oven and recording the temperature by a data logger. This test is completed when the temperature difference between the two thermocouples’ reading disappears (Wang et al.2006; Chen & Chong1998; Chen1999). The reaction rate is expressed by first order kinetics and an Arrhenius expression for the rate constant. The analysis remains valid at temperature higher than 100°C, since water evaporation is ignored in the energy balance (Wang et al.2006).
The crossing point method of Jones requires the measurement of the temperature difference between the centre temperature and the oven temperature (Wang et al.2006), as measured near the sample’s surface. Worden implemented Jones’ method to determine the global kinetic parameters of the cotton impregnated with boiled linseed oil (Worden2011). At low oven temperatures, the crossing point temperature derived by Chen’s method approximates that obtained by Jones’ method (Chen1999). However, the two temperatures diverge as the oven temperature increases. From a theoretical perspective, Chen’s method yields fundamental kinetic parameters, provided that the first order kinetic rate model holds. On the other hand, Jones’ method results in estimates of apparent kinetic parameters that nonetheless could be useful for ranking materials for their tendency to self ignite (Chen1999). See Appendix for the derivation of both methods and more complete discussion of their limitations.
Gross and Robertson employed an adiabatic reactor to determine the kinetic constants, E and A (Gross & Robertson1958). It consists of a Dewar flask, enclosed within a close-fitting cylindrical shell to reduce heat losses and maintain the homogenous temperature inside the furnace. The shell comprises two concentric stainless steel cylinders, equipped with two electric heating elements and an insulating fill, as well as the bottom and centre guard heating elements. The top-guard heating element is located within the Dewar flask plug which is composed of several layers of asbestos board. A thyratron control system permits the adjustment of the guard heater cycle. The copper-constantan thermojunctions, arranged in series, serve to measure the mean temperature-difference between the specimen and the ambient medium (Gross & Robertson1958).
From a fundamental perspective, this method entails no heat transfer through the surfaces of the sample. This simplifies the heat transfer equation to two terms, heat generation and heat accumulation, leading to an integral heat balance expressed in terms of an ordinary differential equation with time as an independent variable. As the kinetic properties (E, A) are obtained for an average temperature of the sample, they convey the meaning of apparent or effective values. Hence the technique yields measurements of similar quality as those of the crossing point method of Jones. Its advantage over the Jones’ method lies in a requirement of a single experiment, at a cost of significantly more complicated experimental equipment to ensure the adiabatic operation of the reactor.
Thermogravimetry-differential thermal analysis (TGA-DTA), or thermogravimetry-differential scanning calorimetry (TGA-DSC), involves heating small samples of materials (in the order of 5 to 10 mg), usually by imposing a constant temperature rise (in the order of 5 to 10°C min-1) and measuring mass loss of the sample and associated heat effects. A gentle temperature ramp allows heat to be transferred to the sample (or removed from the sample, in case of exothermic reactions) on a time scale that is shorter than the sample decomposition. Likewise, a small sample size permits the gaseous reaction products to be removed efficiently from above the sample, preventing or minimising the occurrence of reverse and secondary gas-phase reactions. The presence of reverse reactions makes the system operate close to equilibrium, introducing complications in the data analysis that normally assumes the existence of a forward reaction only (i.e., non-equilibrium conditions). On the other hand, secondary gas-phase reactions may affect TGA measurements by depositing secondary char onto the sample, and may impact the DTA readings due to heat effect of these reactions. It is not often appreciated that DTA or DSC instruments also detect energy released in the gas phase reactions, which occur near the sample surface. The method has been applied to examine the spontaneous ignition of cotton fabrics with and without linseed oil (Khattab et al.1999).
In a typical study, the fabric materials impregnated with linseed oil, with sample sizes of around 10 mg in mass, were placed in the DTA furnace and heated at rates of between 5 and 20°C/min, while the onset of spontaneous ignition was measured by oxygen consumption (Khattab et al.1999). The authors did not report the effect of different gas flow rates and sample mass and therefore the possibility of backward and secondary reactions could not be concluded from these experiments. Particularly, it is uncertain whether the investigators executed their experiments under non-equilibrium conditions. A preliminary study to determine the optimal conditions (flow rate, sample mass) is essential to achieve accurate measurements.
Khattab found that as the linseed oil concentration in the cotton increases, the apparent activation energies of pyrolysis and oxidation decrease, due to hypothesised formation of free radicals from oxidation of linseed oil which then catalyse the pyrolysis reactions of cotton (Khattab et al.1999). Linseed oil oxidation, which incorporates cross-linking reaction, involves oxygen consumption and induces increasing sample mass. Thus, the mass reading of a TGA instrument corresponds to a combined effect of mass-gaining (i.e., oxygen consumption) and mass-losing (i.e., emission of carbon oxides and water) reactions. For this reason, the gravimetric techniques alone are insufficient to probe the spontaneous ignition of linseed oil impregnated on the cellulose materials. These techniques should be accompanied by other methods such as infrared spectroscopy or mass spectrometry (i.e., TGA-DSC-FTIR or TGA-DSC-MS).
Another somewhat dated method to test the spontaneous heating involves a comparison of the temperature histories (i.e., plots of temperature versus time) of two cotton balls (one oily and one as a blank), placed some distance apart from each other inside a light steel tube, known as the Ordway apparatus. The tube is then heated by an external air bath surrounding the tube. Fresh air is supplied to the sample and the two balls receive the same heat. Unfortunately, this method was found not to yield reproducible results (Thompson1928).
Mackey apparatus is a rather old test protocol that involves a jacketed cylinder 10 cm in diameter and 17.5 cm in height, covered on the top and equipped with a thermometer (Khattab et al.1999). It was originally developed for testing the selfheating and ignition of oils utilised in the wood industry (Bowes1984). Two tubes are connected to this apparatus, one extending down into the cylinder and the other upward from the cover. A cotton sample soaked with oil is placed in a wire gauze cylinder and held concentrically in the apparatus. The procedure involves boiling of the water in the bath, inserting the sample into the apparatus and recording the thermometer readings (Thompson1928). The oil is categorised as safe if the temperature does not exceed 100°C in 1 h or 200°C in 2 h.
Theory of thermal ignition and its application to linseed oil
Three models, characterised by different temperature profiles, have been developed to describe a spontaneously-heating system, as illustrated in Figure 4. Semenov, ignoring the temperature differences within the reacting gas mixtures, assumed a uniform temperature profile, neglected the reactant consumption and supposed that chemical reaction followed the Arrhenius temperature dependence. The Frank-Kamenetskii model incorporates the conduction from the material’s surface as the only heat loss mechanism and assumes the equality of ambient and material temperatures at the surface. Within the material, the temperature profile arises as a consequence of chemical heat release, boundary conditions and heat conduction in the bulk. The Thomas model considers both conduction through the solid and convective heat loss from the surface (Drysdale1985).
The basket heating method was developed based on the steady state theory of ignition by Frank-Kamenetskii. The critical condition for the onset of self ignition is represented by a critical dimensionless parameter, δ c , also named as the critical Damköhler number, as defined in Eq. 3 (Drysdale1985; Chen & Chong1998; Hill & Quintiere2000):
Semenov, Frank-Kamenetskii and Thomas’ models assume a single step global reaction (i.e., no competing or parallel reactions), no reactant consumption, no effect of oxygen availability, and constant thermal properties of the system (Drysdale1985; Worden2011). Thus the models apply to systems characterised by simple low-temperature oxidation chemistry, or to systems for which the global one-step kinetics provide a reasonable approximation to the actual chemical behaviour. This is not the case for self-heating of lignocellulosic materials soaked with linseed oil.
A typical approach is to obtain global kinetic parameters (E and A) from the crossing-point method of Chen (Chen & Chong1998) (Appendix) or from an adiabatic reactor, as the one used by Gross and Robertson (1958). These values are then substituted into the classical expression of Frank-Kamenetskii to yield a relationship between the characteristic length of a system and the critical ambient temperature
Worden, as well as Gross and Robertson, calculated the global kinetic parameters for the oxidation of cotton soaked with linseed oil. Worden studied the behaviour of boiled oil (i.e., oil that includes cobalt catalyst to accelerate drying), whereas Gross and Robertson investigated the performance of raw linseed oil. It is not surprising that the results are quite different. But, as one would expect, Worden obtained significantly lower estimates of the global activation energy and substantially higher evaluation of the global pre-exponential factor (Table 4). Two considerations should be pointed out. Firstly, the researchers applied different methodologies, Gross and Robertson used the adiabatic reactor, whereas Worden relied on the Jones’ crossing-point method (see Appendix). This method is inferior to that of Chen. Secondly, Worden’s results have convincingly demonstrated the failure of the classical self-ignition theory for cotton soaked with linseed oil. In particular, Worden found both global kinetic parameters (E and A) to vary, whereas they are constant in the classical treatment. This indicates that the simple first order kinetic model employed in the classical theory is inconsistent with the actual behaviour of the material. This conclusion also follows from large differences between measured and predicted self-ignition temperature for cotton linters provided in Table 2 of the paper of Gross and Robertson (Gross & Robertson1958).
There is a kinetic compensation effect in the results of Worden (Worden2011); i.e., a linear relationship between E and log (A ΔH c) of the form of ln(A ΔH c) = constant + E/(R T), with the isokinetic temperature of 361 K (Figure 5). This temperature signifies approximately the same reaction rate for the three sets of Worden’s measurements of A ΔH c and E, and the compensation itself means that the effect of the change in the activation energy is compensated by the change in A ΔH c. There is no generally accepted mechanistic explanation of the compensation effect, although it stands to reason to suggest that the effect arises due to availability of more than one radical species to bind with oxygen. A further investigation of this effect is required to elucidate its explanation for the present system and to confirm its existence by statistical tests.
The NFPA Handbook (Drysdale1985) includes linseed oil absorbed on to the fibrous combustible materials such as cotton rags in the list of materials with the tendency of spontaneous heating. Cotton fabric materials soaked in linseed oil, which contains dryers, can auto-ignite about six to eight hours after the rags start to dry, in a process preceded by smoke and rancid odour (Howitt et al.1995).
The material’s low surface to volume ratio encourages self-ignition, as this limits heat losses, facilitating the material’s temperature rise. This ratio is inversely proportional to a characteristic dimension (radius) of the material; the larger this characteristic dimension, the lower the surface to volume ratio and higher the propensity to self-heat. The ignition occurs once the size of a material exceeds its critical radius, with smaller values of critical radius indicating increasing risk of self-ignition. For example, at 100°C, wood fibreboard, cork and cotton soaked with raw linseed oil possess critical radii of 0.5, 0.55 and 0.017 m (Gross & Robertson1958), while coal exhibits self-heating only under bulk storage conditions. This supports the previous statement that linseed oil applied onto the cotton induces a greater tendency of the resulting material to self-heat.
Table 5 compares the physicochemical properties, which govern the self-heating, of several materials to cotton with linseed oil content of 16.7%. Clearly, the activation energies of other materials remain much higher than that of cotton impregnated with linseed oil, with the exception of cork which displays a similar value. This further reinforces the particularly hazardous nature of cotton soaked with linseed oil, in comparison with other materials. Consistent with the findings for cotton, Napier and Vlatis (Napier & Vlatis1981) reported a decrease in the minimum ignition or self-heating temperature of the mixture of beech saw dust and raw linseed oil with larger sample sizes, an increase in oil content in the range of 0–50% and a decrease in particle size. However, the presence of moisture in the material increases the self-heating temperature. These effects are not included in the classical theory of self-ignition, suggesting caution in applying this theory to lignocellulosic materials impregnated with linseed oil.
Measurement of oxidation of linseed oil
Different methods have been applied to investigate the oxidation reactions of linseed oil. One of these methods comprises spreading the oil to achieve a given film thickness on selected supports, and then imposing different oxidation conditions on the samples. These conditions may involve thermal treatment in a forced-air circulation oven at 80°C, natural ageing in the laboratory at room temperature and photo-ageing in a high speed exposure unit with a xenon lamp. The final step comprises the analyses of chemical changes during the treatments, as described later in the review (Lazzari & Chiantore1999). The difficulty of this method is to maintain the same accurate film thickness to collect reproducible quantitative results.
Fjällström et al. employed test chambers made of glass jars, equipped with metal lid and teflon disc, immersed into a water bath to control the temperature, and preconditioned air flowing through the chambers. The authors controlled the air humidity, the light intensity and the bath temperature, and measured the variation of temperature in the chambers. The authors placed linseed oil paint applied on the glass plates in the chamber and the gaseous products were passed through the silica gel impregnated with 2,4-dinitrophenylhydrazine and then analysed with high performance liquid chromatography (HPLC) (Fjällström et al.2002). Increasing temperature and humidity enhanced total emission of aldehydes, while artificial light and air exchange rate produced no significant effects. This method required complex and time consuming procedures for sample preparation prior to analysis, involving solvent washing, evaporation, decantation and desorption of adsorbent with solvent.
Thompson reported that absorption of oxygen is an indicator of the spontaneous heating of oil. A brass bottle with a rubber stopper was immersed in a bath of boiling water, equipped with a thermometer. The net amount of oxygen absorbed was measured by a pressure decrease (Thompson1928). However, Thompson’s methodology needs to be treated with caution since the pressure reading corresponds to the combined effect of the oxygen absorption and release of gaseous products of the oxidation reactions.
Thermal degradation of oil samples has been studied by differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). DSC has been applied to examine the kinetic parameters such as activation energy, preexponential factor and rate constant (Litwinienko & Kasprzycka-Guttman1999) of oxidation of linseed oil. The thermal decomposition of raw linseed oil analysed by DSC consisted of three steps, an exothermic process related to oxidation between 150 and 250°C, the first stage of oxidative decomposition between 250 and 400°C and the main processes of decomposition above 400°C (Lazzari & Chiantore1999). An exothermic peak at 155°C and degradation, which started at 300°C, were also observed by Suryanarayana et al. during curing of linseed oil (Suryanarayana et al.2008). The peroxide formation may occur in the first step, followed by the decomposition of peroxides into radicals. The reaction of these radicals with unsaturated compounds is an exothermic process, accompanied by partial mass loss due to the fragmentation reactions (Lazzari & Chiantore1999).
Tuman et al. used differential scanning calorimetry (DSC) to study the autoxidation of linseed oil catalysed by manganese and zirconium dryer, as well as titanium alkoxide. The authors reported the manganese dryer catalysed the autoxidation step at the top surface of the coating only, while the zirconium dryer appeared active throughout the film (Tuman et al.1996). This observation contradicts that of Mallégol et al. (Mallégol et al.2000) and Meneghetti et al. (Meneghetti et al.1998) who reported that a zirconium dryer exhibits no influence on the curing of linseed oil without a primary dryer.
The oxidation reaction of linseed oil can also be performed with the method described by Niczke et al. (Niczke et al.2007). These authors carried out the oxidation of rapeseed oil methyl ester in a flask at 200°C with the air flowing for 25 h. The liquid products were collected for analysis every 5 h and the volatile products were trapped in a scrubber with trichloroethylene for analysis employing FTIR, H-NMR and GC-MS as described later in this paper (Niczke et al.2007).
Additionally, a flow reactor served to study oxidation of linseed oil, as illustrated in Figure 6 (Juita et al.2011a; Juita et al.2010a; Juita et al.2010b; Juita et al.2011b). The study incorporated glass or lignocellulosic substrates impregnated with linseed oil, corresponding to the cases of spontaneous combustion of rags soaked with linseed oil. The reactor, made of copper, 11.8 cm in length and 0.94 cm in diameter and located inside a Thermoline oven, operated at temperature of either 80 or 100°C. Mass flow controllers measured the flowrate of oxygen and nitrogen gases prior to entering the reactor. A particulate filter enhanced the mixing of those gases (in equimolar proportions). The feed gases were subsequently preheated in copper coil tubing in the reactor oven, allowing the temperature of the feed gases to equilibrate with the oven temperature. A micro gas chromatograph enabled the quantitation of the gaseous products from the output of the reactor. Gas sampling bags collected and stored the reaction product gases for identification on a Fourier transform infrared spectrometer and a gas chromatograph–mass spectrometer.
Another experimental technique able to provide high quality kinetic measurements is a jet stirred reactor (JSR). A typical JSR consists of four capillary jets that eject incoming gases at high velocity (on the order of 50 to 100 m/s) into a spherical reactor vessel inducing thorough stirring of the reactor’s content, allowing one to assume perfect mixing. The mathematical description of the reactor involves a system of algebraic equations, which are more readily solved than a system of ordinary differential equations or partial differential equations required for turbulent (plug) and laminar flow reactors, respectively. JSR allows obtaining high quality measurements of formation of chemical species needed for developing kinetic models of oxidation. A study on the oxidation of linseed oil in the gas phase in a JSR in the temperature window from 550 to 750 K may yield significant insights into the mechanism of hydroperoxide formation, suggesting a new avenue of inquiry for future research. This temperature window covers the peroxy reactions that govern the formation of cool flames. Similar reactions operate in spontaneous ignition of linseed oil, though at lower temperatures. In previous studies, the measurements obtained from the experiments involving JSR were applied to validate kinetic models of oxidation of n-butanol (Sarathy et al.2009), methyl octanoate-1-butanol (Togbe et al.2010a n-decane (Dagaut & Cathonnet2006)), mixture of n-decane and n-propyl benzene as surrogate fuels of kerosene (Dagaut & Cathonnet2006), and methyl octanoate-ethanol as surrogate fuel of biodiesel-bioethanol (Togbe et al.2010b). From this perspective, an initial investigation, to gain insights into the oxidation of linseed oil, may involve a study on ethyl linoleate or linoleic acid.
Figure 7 illustrates a jet-stirred reactor (JSR), made from a fused-silica sphere, 4 cm in diameter. Four nozzles, 1 mm in diameter, inject the gases into the reactor inducing their mixing. Fuel enters the reactor together with nitrogen via an atomiser-vaporiser that is maintained at 200°C. A thermocouple (0.1 mm Pt-Pt/Rh 10%) monitors the temperature along the vertical axis of the reactor (Dayma et al.2011; Dagaut et al.1986).
A laminar flow reactor presents an alternative approach to JSR to elucidate the oxidation reaction of linseed oil at low temperatures, with literature examples including investigations of the pyrolysis and oxidation of catechol, captan, folpet and alpha-cypermethrin (Chen et al.2011aChen et al.2011b; Summoogum et al.2011; Thomas et al.2007; Wornat et al.2001). A typical experimental apparatus comprises a vaporiser, isothermal flow reactor and a product collection system as shown in Figure 8. The sample was loaded into a vertical tube and placed in an isothermal oven that functioned as a vaporiser. Carrier gas entrained the vapour and flowed to the high temperature laminar flow reactor where the pyrolysis or oxidation reactions took place. The reactor constituted a central constant-temperature zone (T 2 in Figure 8) of an alumina tube affording residence time of 0.3 to 1 s at a temperature in excess of 300°C. The gaseous products were trapped in a sampling bag, while the condensed-phase products were collected in separate experiments by activated charcoal adsorbent and XAD-2 resin cartridges (Chen et al.2011a; Thomas et al.2007). Charcoal is no longer recommended for this application, as its strong adsorption properties result in low recoveries of some VOCs.
Analytical methods to study the oxidation chemistry of linseed oil
Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), Raman spectroscopy, UV–vis spectrometry and chemiluminescence have been implemented to investigate the structural changes of the active compounds in the linseed oil (i.e., oleic, linoleic and linolenic acids) during their oxidation. Lazzari et al. investigated the degradation of linseed oil, by natural and accelerated weathering, by means of FTIR (Lazzari & Chiantore1999). The results of their FTIR analysis on linseed oil film, which had been treated isothermally at 80°C, indicated that, after an induction period of around 4 h, the hydroxyl groups increased in abundance up to a constant value, and the double bonds commenced to disappear, indicating that significant chemical changes occur between 4 and 8 h into the drying process (Lazzari & Chiantore1999). Intensity of specific infrared bands, as measured by FTIR, provides a convenient method for monitoring the sample consumption. For example a cis C = CH vibration at 3010 cm-1 (Stava et al.2007; Oyman et al.2004; Warzeska et al.2002), or emergence of a particular functional group, such as hydroperoxide, may be determined by monitoring the absorption of the infrared signal at 3472 cm-1 (Meneghetti et al.1998). These results indicate that FTIR provides an excellent means to identify the functional groups present in the oil. However, the method cannot determine the structure of the reacting species, and appears to have no capacity to quantitate the oil’s triacylglycerol content.
Mallégol et al. observed that the variation in the magnitude of the easily-detected vibrational bands of conjugated compounds gauges the rate of oxidation reaction, which exceeds that of the saturation reaction, in the presence of dryer (Mallégol et al.2000). The epoxidation reaction of linseed oil manifests itself by the disappearance of the 3010 cm-1 band and the emergence of oxirane rings indicated by the appearance of two bands at 825 and 845 cm-1 (Martini et al.2009).
FTIR analysis of gaseous products of linseed oil oxidation has identified the presence of carbon dioxide in the spectral region of 2240 to 2411 cm-1, carbon monoxide in the region of 2060 to 2220 cm-1, formic acid in 1060 to 1144 cm-1, propionaldehyde in the band of 828 to 867 cm-1, acrolein in 900 to 1000 cm-1, whereas between 1700 and 1800 cm-1 the spectrum displays strongly overlapping bands of formic acid, acetaldehyde, propionaldehyde, acrolein and crotonaldehyde. Similarly, propionaldehyde, crotonaldehyde and ethane overlap between 2500 and 3100 cm-1 (Juita et al.2010a).
Time-resolved FTIR (TR-FTIR) and attenuated total reflectance (ATR) techniques can assess the chemical changes during the oxidation of linseed oil, as demonstrated by their application to oxidation of ethyl linoleate, a model compound for linseed oil. For example, applications of TR-FTIR yielded understanding of the behaviour of different pyrazoles as anti-skinning additives (to prevent the formation of solid skin during drying) in the oxidation of ethyl linoleate by bis(acetylacetonato)cobalt(II) [Co(acac)2] (Tanase et al.2004) and have unravelled the effect of cobalt dryer and ferrocene derivatives on the induction time (Stava et al.2007); that is, the time before significant changes are observed in the concentration of ethyl linoleate.
The results of the H-NMR spectroscopic analysis of Niczke et al. (Niczke et al.2007) have demonstrated that the relative number of methyl protons (δ = 0.86 ppm) remains unaffected by the oxidation of rapeseed oil methyl ester. However, the relative concentration of protons (δ = 5.3 ppm) attached to alkenyl (−HC = CH−) carbons reduces dramatically from 2.6 in rapeseed oil methyl ester to 1.1 in liquid products of oxidation of rapeseed oil methyl ester and 0.6 in volatile products of oxidation of the ester. Niczke et al. report a less significant decrease in methylene protons (δ = 1.96 ppm) attached to α carbons (i.e., −CH2 − HC = CH−), with changes in relative concentration from 4.0 (unoxidised ester) to 2.4 (liquid products) and 1.5 (volatile products) (Niczke et al.2007). In comparison, protons (δ = 1.57 ppm) attached to β carbons display relative decrease from 20.6 to 18.4 and 12.6. These results demonstrate a preferential cleavage of the bonds between − CH2− and − CH2 − HC = CH − groups during the formation of gaseous products in the oxidation process, and removal of protons attached to alkenyl carbons both during the oligomerisaton and formation of gaseous products. The H-NMR spectra of linseed oil are illustrated in Figure 9. The chemical changes diagnosed by H-NMR during oxidation of ethyl linoleate (EL), catalysed by combination of ascorbic acid 6-palmitate (AsA6p) and iron(II)-2-ethylhexanoate (Fe-eh), include a decrease in intensity of the cis-double bonds (a), bisallylic methylene (b) and monoallylic methylene (c), the formation of conjugated double bonds at 5.5-6.6 ppm, allylic methine protons (a1) at 4.3 ppm, as well as growth of conjugated EL hydroperoxides at 7.9 and 8 ppm (Miccichè et al.2006), as shown in Figure 10.
H-NMR was also applied to probe the epoxidation reaction of linseed oil, indicated by the disappearance of vinylic hydrogens at 5.3 ppm and the appearance of epoxy groups at 2.9-3.1 ppm (Martini et al.2009). NMR spectroscopy is a useful method for identification of very fine structural components, however, it requires costly instrumentation and significant expertise to interpret the spectra.
Raman spectroscopy complements FTIR spectroscopy by identifying the symmetric vibrational modes, while FTIR measures the absorption of infrared energy by the asymmetric vibrational modes and polar groups (Muik et al.2005). Thus, Raman spectroscopy detects the symmetric diatomic molecules such as nitrogen and oxygen which cannot be measured by infrared spectroscopy, but fails to detect asymmetric vibrational modes. Raman spectroscopy provides chemical spectra that can probe the chemical changes during oxidation of linseed oil, which are reflected in changes of the magnitude of the cis C = C-H asymmetric stretch (3012 cm-1) (Oyman et al.2004).
Oyman et al. used Raman spectroscopy to compare changes in the abundance of double bonds during oxidation of linseed and tung oils. For linseed oil, the abundance of the non-conjugated double bond decreased as the oxidation proceeded and non-conjugated double bonds converted to conjugated double bonds (Oyman et al.2005a). In contrast, the abundance of the conjugated double bonds in tung oil decreased during oxidation, and, after 90 h of an experimental run, only a small peak signified the residual amount of conjugated trans double bond left (Oyman et al.2005a). By means of a Raman spectrometer, Muik et al. analysed aldehydes and epoxy compounds, such as trans-9,10- and cis-9,10-epoxystearic acids as the oxidation products of oleic acid (Muik et al.2005). They reported a weak band at 1724 cm-1 to relate to the C = O stretching of hexanal and a very strong band at 1641 cm-1 to correspond to the conjugated C = C stretching of the trans-trans-2,4-decadienal, the major decomposition product of linoleate (Muik et al.2005).
UV–vis spectroscopy detects the formation of ligand complexes of cobalt, an active catalyst for the decomposition of hydroperoxides in ethyl linoleate (Tanase et al.2004). UV–vis has been also implemented to observe the conjugated diene structure at the absorption maximum of 232–232.5 nm (Belhaj et al.2010; Hendriks et al.1979). The concentration of conjugated dienes in the oil samples increases during the storage (Belhaj et al.2010), indicating that oxidation proceeds during this period. In its application to oxidation of linseed oil, UV–vis yields rapid qualitative information. However, it requires the determination of absorption coefficients to produce quantitative measurements. This however may not always be possible for complex mixtures of chromophores.
Chemiluminescence provides another approach to analyse the lipid peroxidation. Rolewski et al. reported that hydroperoxides produced during oxidation of linseed oil at 60°C increased almost linearly during 2 h, then reached a constant value whence finally their concentration slowly decreased. This method affords fast measurements with higher sensitivity compared to the determination of peroxide value using iodometric titration. However, carotenes, flavones and riboflavin, if present in oil, confound the measurements (Rolewski et al.2009).
Chromatographic and mass spectrometric techniques
Chromatographic techniques serve to identify and quantitate the oxidation products of linseed oil. These techniques comprise micro gas chromatography (μGC), high performance liquid chromatography (HPLC), gas chromatography–mass spectrometry (GC-MS), solid phase micro extraction (SPME) combined with GC-MS and size exclusion chromatography (SEC). The application of each method is described below.
Micro gas chromatography (μGC) quantitated the emission of gaseous products during oxidation of linseed oil, with carbon dioxide as the major product. The concentration profile of each species comprises three stages: the first period whereupon the concentration rises significantly to reach a maximum value, followed by a sharp decay in the second stage and finally the concentration declines slowly (Juita et al.2011a).
High performance liquid chromatography (HPLC) has been deployed to investigate the aldehyde products of paint oxidation. A silica gel cartridge impregnated with 2,4-dinitrophenylhydrazine (DNPH) sampled the products, with authentic reference substances employed to identify them (Fjällström et al.2002). Liquid chromatography-mass spectrometry/mass spectrometry analysis (LC-MS/MS) confirmed the structure of the compounds. Methanal, ethanal, propanal, pentanal and hexanal constituted the most abundant aldehydes emitted from the paint oxidation at ambient temperature (Fjällström et al.2002), and their emission occurred mostly during the first day of the oxidation process.
In general, HPLC affords analysis of a larger range of compounds than is possible with GC. However, HPLC is a more costly technique than GC owing to the solvent usage and at present cannot match GC when considering resolution and sensitivity. These considerations make GC the preferred technique for analysis of volatile analytes.
The methanolic extracts of paint samples of different historical ages (5, 26 and 373 year old), analysed by GC-MS, revealed that younger paint film contained more monounsaturated fatty acid and less diacids compared to older paints. This means that the oxidation process continues in paints over hundreds of years. The unsaturated fatty acids are oxidised leading to the higher amount of diacids in older paints. Analysis of 5-year old stand oil film identified short chain fatty acids (C7-C10), diacids (C7-C11), saturated long chain fatty acids (C16-C18, C20-C22), a cyclic C18 fatty acid and some unsaturated and/or oxidised C18 fatty acids, in the extracts. In addition, the analysis detected monounsaturated C18 fatty acids, but no doubly and triply unsaturated fatty acids, although all acids had been initially present in high concentrations in the oil (van den Berg et al.2002).
Solid phase microextraction (SPME) method has been employed for the analysis of volatile components in food, oils, water, soil (Havenga & Rohwer1999) and in environmental samples. The advantages of this method include solvent-free operation, small quantity of sample needed, low cost (Ribeiro et al.2008) and fast sample preparation compared to conventional liquid-liquid extraction or soxhlet extraction (Eriksson et al.2001). This is a simple method which incorporates sampling, extraction, concentration, and sample introduction (Contini & Esti2006), replacing the tedious sample pretreatment and extraction process required for classical techniques.
Different types of fibre have been found suitable for analyte absorption, including 100 μm polydimethylsiloxane (PDMS) for samples of virgin olive oil (Jiménez et al.2004; Baccouri et al.2007), 30 and 50 μm divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) for linseed oil (Wiesenborn et al.2005; Krist et al.2006), oil in water emulsion and marine salt (Beltran et al.2005; Silva et al.2010), rapeseed oil (Jeleń et al.2000) and sunflower oil (Guillen & Goicoechea2008), 75 μm CAR/PDMS for tonalin oil and safflower oil (García-Martínez et al.2009), 100 μm PDMS and 85 μm polyacrylate (PA) for olive oil (Ribeiro et al.2008). Among the four types of fibres, PA, PDMS, carbowax/divinylbenzene (CW/DVB) and DVB/CAR/PDMS, the last one was reported to provide the best detection of analysed compounds, followed by CW/DVB which afforded the detection of all compounds even with smaller peak areas. PDMS had lower extraction capacities and PA showed the lowest extraction ability (Jeleń et al.2000).
The critical aspect in solid phase microextraction is the establishment of an equilibrium condition between gaseous and adsorbed species. The conditions that influence the establishment of the equilibrium include the temperature and duration of the adsorption experiments, fibre type, as well as the size of the compounds and their concentration. From this perspective, researchers explored different conditions to attain the equilibrium, for instance, varying sampling temperature from ambient to 60°C and absorption time of between 15 min and 10 h (Havenga & Rohwer1999; Ribeiro et al.2008; Jiménez et al.2004; Baccouri et al.2007; Wiesenborn et al.2005; Krist et al.2006; Beltran et al.2005; Silva et al.2010; Jeleń et al.2000; Guillen & Goicoechea2008; García-Martínez et al.2009). The sampling typically proceeds at 60°C for 15 to 20 min.
The SPME-GC-MS method has been exploited for the quantitation and identification of VOC in the headspace of raw and boiled (with metal dryer content) linseed oil. Several types of VOC were identified, including saturated and unsaturated aldehydes, ketones, alcohols, carboxylic acids and furans. The concentration of propanal, hexanal, 2-pentenal, 1-penten-3-ol, 2,4-heptadienal, 2,4-decadienal, 3,5-octadien-2-one, acetic acid and hexanoic acid increased during 6 h oxidation at 80°C . The emission of VOC from raw linseed oil is significantly lower than from boiled linseed oil (Juita et al.2011c), owing to metal catalysed reactions in the latter.
Krist et al. utilised SPME technique to analyse volatile compounds in the headspace of three types of linseed oil, two of them produced by pressing flax seeds at room temperature (Lower Austria, and Saxony, Germany), while the third (Styria, Austria) was obtained by pressing the seeds, previously heated at 60°C for 30 min, at room temperature. VOC detected from all types of oil comprised acetic acid, trans-2-butenal, trans-2-pentenal, hexanol, trans,trans-2,4-hexadienal, 2-pentylfuran, trans,trans-3,5-octadiene-2-one and nonanal. Some species, including 2-butanol, ethylbenzene, heptanal, benzaldehyde, octanal, decanal, were only identified in linseed oil from Germany. The differences in the composition of VOC appear to originate in plant varieties, cultivation and extraction conditions (Krist et al.2006).
Volatile organic compounds produced from linseed oil have also been studied by thermal desorption gas chromatography – mass spectrometry combined with olfactometry odour recognition (TD-GC-O/MS); the latter performed by combination of expert and non expert panellists who had been requested to report the first perception of an odour (Clausen et al.2008). A detection frequency method was implemented to measure the odour intensity (Clausen et al.2008), by counting the number of panellists who detect an odour active VOC. This method routinely serves to detect and identify odour active VOC, in the perfume and food industries, and to evaluate odour from paint emission in indoor environment (Clausen et al.2008).
Literature reports the application of combined matrix-assisted laser desorption/ionisation -reflectron-time-of-flight (MALDI-RTOFMS) and electrospray ionisation (ESI-MS/MS) to analyse the triacylglycerol composition of linseed oil. Figure 11 demonstrates the spectra of triacylglycerols in linseed oil generated by MALDI-MS. The experiment determined that more than 50% of triacylglycerols comprise one to three linolenic acid groups (Krist et al.2006) in linseed oil.
Two dimensional gas chromatography time-of-flight mass spectrometry (GC × GC-TOFMS) has been developed to analyse the volatile and semi volatile compounds from complicated mixtures, employing separation based on the volatility in the first dimension and polarity in the second dimension (Silva et al.2010), including the identification of volatile compounds in virgin olive oil (Kanavouras et al.2005). In this technique, the sample is initially separated in the first column, and the eluent collected over short time periods and then injected into the second column. This method can resolve the problem of co-elution encountered in one-dimensional chromatography which limits the automatic species identification by library searches (Silva et al.2010). Figure 12 illustrates that the co-eluting compounds of similar volatilities on a non-polar column can be well separated on a polar column due to their different polarities (Silva et al.2010). Clearly, two dimensional GC significantly improves the analysis. Kanavouras et al. reported that a combination of SPME (using PDMS-DVB fibres) and GC-TOFMS seems to be faster and simpler compared to dynamic headspace thermal desorption (DHS-TD)/GC-MS using Tenax TA trap; however, the latter displays higher efficiency in terms of the number of compounds separated (Kanavouras et al.2005).
Size exclusion chromatography (SEC) is particularly suitable to analyse the product of the polymerisation reaction of linseed oil involving its active constituents. It was employed to observe the polymerisation of ethyl linoleate in the presence of Co(acac)2/additive mixture (Tanase et al.2004) and the oligomerisation of ethyl linoleate catalysed by 1,4,7-trimethyl-1,4,7-triazacyclononane (MnMeTACN/HMTETA) (Oyman et al.2004). During the first day of drying, oligomer formation was observed relatively quickly for the oxidation reaction of ethyl linoleate in the presence of cobalt/calcium/zirconium dryers. No significant change in the oligomeric distribution occurred on further drying (Muizebelt et al.1994). This method quantitates the abundances of hydroperoxides, dimers, trimers and higher oligomers, providing information about rates of cross linking reactions (Wu et al.2004). By applying SEC, Lazzari et al. identified the peak eluting at 32.5 min as corresponding to the formation of dimers. These researchers reported that the weight of the sample increased up to a maximum of 7% after 20 h of oxidation at a constant temperature of 80°C (Lazzari & Chiantore1999). This increase could be related to the incorporation of oxygen that manifests itself by fast accumulation of insoluble fractions as a result of cross-linking reactions. The formation of small amounts of volatiles caused the weight to decrease after 20 h treatment (Lazzari & Chiantore1999).
Different paints emit distinct species of aldehydes. For instance, paints rich in linoleic and linolenic acids produce mostly hexanal (Hancock & Leeves1989) and propanal (Serfert et al.2009), respectively, as analysed by GC-MS techniques (Fjällström et al.2002). Nonanal arises as the major volatile species produced during oxidation of lipids present in oleic acid (Beltran et al.2005; García-Martínez et al.2009). Oils rich in conjugated linoleic acid, such as tonalin oil, produce a combination of hexanal and heptanal as the two main oxidation products, with hydroperoxides as minor products (García-Martínez et al.2009).
The presence of 2-pentenal, hexanal, 2-heptenal, 2-octenal, octanal and nonanal defines the rancidity of fats, while hexanal, octanal and 2,4-decadienal characterise the rancidity of food (Jiménez et al.2004). Hexanal comprises the most abundant compound found in fresh rapeseed oil obtained by cold pressing and after 5 days of storage at 60°C, while, after 10 days, 2-heptenal commenced to dominate the aldehyde distribution (Jeleń et al.2000). For this reason, the ratio of hexanal to nonanal has been suggested as an index describing the quality of olive oil (Mildner-Szkudlarz et al.2003).
The secondary degradative oxidation compounds could be classified as acids, alcohols, esters, hydrocarbons, furan and carbonylic derivatives (Guillen & Goicoechea2008). The main acids detected in the headspace of the oil samples include formic, acetic and hexanoic acid, while hydrocarbon compounds emitted comprise saturated, monounsaturated, diunsaturated and cyclic hydrocarbons (Guillen & Goicoechea2008).
Table 6 assembles analytical techniques employed to study the oxidation of linseed oil, including types of chemical reactions investigated, and compounds detected.
Parameters and indices assessing progress of oxidation of linseed oil
Iodine, peroxide, saponification and hydroxyl values constitute common parameters describing the reactivity and chemistry of the components of fats and oils (Knothe2002). The iodine value measures the amount of unsaturation of fats and oils. The saponification value relates to the average molecular weight or chain length of the compound. Finally, the hydroxyl value yields the abundance of species containing hydroxyl group, providing an estimate of the degree of oil oxidation.
A small increase in iodine number of oils may indicate a significant self-heating potential of the oil, as the relationship between the iodine number and the content of double bonds appears non-linear (Howitt et al.1995). Linseed oil has a saponification value of 189–195 and an iodine value of more than 177, respectively. This iodine value significantly exceeds that of palm oil, 50–55, peanut oil, 80–106, rapeseed oil, 94–120, cottonseed oil, 99–199, fish oil, 109 (Knothe2002; Pocklington1990) and therefore indicates the highest tendency of linseed oil to self-heat over other types of oil. Iodine values decline during oxidation of linseed oil (measured in bulk), indicating the consumption of double bonds during the reaction, with the rate constant for overall disappearance of double bonds of 0.030 ± 0.007 h-1 at 80°C (Juita et al.2011d).
Peroxide value directly measures the total amount of peroxide compounds present in the oil, yielding its propensity for further oxidation reactions. This value reflects the net rate of formation and decomposition of the peroxides. In one study, the peroxide value increased up to a maximum of about 500 mmol (kg oil)-1 within 5 h of oxidation at 100°C. The rate of the peroxide decomposition increases as the temperature rises, resulting in the lower peroxide value at higher oxidation temperatures (Juita et al.2011d).
Lazzari et al. compared the hydroxyl index of linseed oil as a function of treatment conditions. The hydroxyl index achieved a constant maximum value after an induction time of around 200 h for linseed oil exposed to indoor laboratory conditions, 4 h for treatment at 80°C and no induction for photo-aged linseed oil. This suggests that the photo-ageing treatment induces the highest degree of oxidation (Lazzari & Chiantore1999).
Allylic position equivalent (APE) and bis-allylic position equivalent (BAPE) have been developed as an alternative to the iodine value since this value does not distinguish the differences in the rate of oxidation; all double bonds are considered as having equal reactivity toward oxidation. APE and BAPE account for the number of reactive positions in oxidation (Knothe2002).
where ap x is the number of allylic positions in a specific fatty acid and A Cx is the percentage of each fatty acid in a mixture (Knothe2002).
Anisidine value (AV) gauges the amount of high molecular weight saturated and unsaturated carbonyl compounds in the secondary oxidation products from oxidation of linseed oil (Guillén & Cabo2002). The method employs spectrophotometric measurement at 350 nm probing both saturated and unsaturated carbonyl compounds (Muik et al.2005). Furthermore, the conjugated dienes and trienes are observed at 232–234 nm and 268–270 nm (Muik et al.2005; Guilén & Cabo2002), respectively. The abundance of conjugated dienes has been suggested as a parameter for assessing the primary oxidation of lipids (Lee et al.2007). Figure 13 illustrates typical histories of the anisidine value during the oxidation process; a slow initial increase, followed by a significant increase, especially for the rapeseed oil (Guillén & Cabo2002). Anisidine value remains to be collected and interpreted for linseed oil. In our view, measurements of the anisidine value could provide valuable assessment of the formation of carbonyl compounds during oxidation, possibly allowing differentiating the propensity to self-heat of various linseed oils.
Oxygen uptake represents another suitable parameter to monitor the oxidation reaction of linseed oil, usually performed with a luminescence oxygen analyser equipped with fibre optics (Oyman et al.2005a; Oyman et al.2004). This type of oxygen analyser measures the quenching of luminescence caused by collisions between oxygen and luminescent dye molecules in the excited state. The oxygen uptake increased sharply up to 20 h during oxidation of linseed oil in bulk at room temperature in the presence of Co(II)-2-ethylhexanoate (Co-EH) then declined to a steady state level (Oyman et al.2005a). Fibre optics-based measurements require a dark environment to prevent the ambient light being collected by the sensor (Wofbeis1987). This effect can be corrected by applying optical isolation, although this slows down the response time by 4 s to about less than 10 s. A luminescence analyser is particularly suitable for quantitating oxygen dissolved in liquids. On the other hand, a paramagnetic oxygen analyser provides very precise measurements of the changes in oxygen concentration in the gas phase. This analyser has been applied with success to monitor oxygen uptake for self-heating materials, to derive kinetic rate parameters, especially for coal (Wang et al.1999; Wang et al.2002).
Film hardness provides an index to appraise the drying of linseed oil, normally measured with a pendulum-damping test (Miccichè et al.2005a). The test measures a number of pendulum swings required for dampening the amplitude of the pendulum oscillations, to a defined level. Harder films afford more swings. This method yields rapid evaluation of the effect of additives on the hardness of a film coating. The addition of ferrocene derivatives to the cobalt dryer, as ingredients of alkyd paints, can reduce the period needed for the paints to reach a certain hardness value (Stava et al.2007). Therefore the usage of ferrocene derivatives can reduce the content of the cobalt dryer in coatings.
The Braive and BK drying recorder is an instrument frequently employed in the coatings industry to measure the drying performance of a paint film operated with different lengths of a test strip; hence the two names used to denote it. This recorder consists of hemispherically ended needles, moving along the length of the test strip, which is made from plain glass and coated with a thin film. The drying characteristics of paint film are classified into four stages: the paint flows together (wet-edge time), the paint begins to polymerise (dust-free time), surface dries and finally lines become no longer visible on the film (total drying or through drying time) (Miccichè et al.2005a). However, Klaasen et al. classified the drying performance into three phases: open time (a scratch line traced by a pin closes up), dust free time (pin leaves a visible scratchy line, partially closed) and tack free time (a scratch line does not close) (Klaasen & van der Leeuw2006). Clearly, assessing the drying of film suffers from subjective evaluation.
Measurement of radicals involved in oxidation of linseed oil
EPR (electron paramagnetic resonance), also known as ESR (electron spin resonance), spectroscopy has been used by many researchers to study the radicals involved in the oxidation reaction of linseed oil. Several variables such as hyperfine structure, splitting factor (g value) and line shape in the EPR spectrum afford identification of a radical (Halliwell & Gutteridge2008). For instance, this technique has been implemented to study the reaction of alkyl radicals with oxygen to form peroxyl radical (Swern1972), occurring in the oxidation reaction of linseed oil. In general, peroxyl radicals exhibit very long half-life of up to 7 s in comparison with hydroxyl (10-9 s) and alkoxyl (10-6 s) radicals.
The stability and reactivity of radicals involving catalysts have been investigated through the formation and disappearance of the radicals obtained by the reaction of Co(acac)2 with t-BuOOH in the presence of pyrazole ligands as anti skinning additives (Tanase et al.2004). The stable coordinated radical, [CoIII(acac)2(ROO•)L]+, produced during this reaction inhibits the radical chain reaction in the drying of paint; where L denotes a neutral donor ligand such as pyrazole and 3,5-dimethylpyrazole. EPR analysis indicates that the stability of these complex radicals depends on the molar ratio between cobalt and an organic additive L (Tanase et al.2004).
The EPR method has also been employed to study the kinetics of free radical reactions in the low temperature autoxidation of triacylglycerols. Fremy’s salt was used to determine the EPR parameters such as g values and hyperfine splittings and the spin intensity was measured by using cupric sulfate as a standard (Zhu & Sevilla1989). The major species present in the EPR spectra included allylic and pentadienyl radicals in trilinolein and trilinolenin samples, respectively. Peroxyl radicals were formed after warming the lipid sample to 105 K and stopped at 135, 132 and 127 K for triolein, trilinolein and trilinolenin, respectively, suggesting that the most stable peroxyl radicals tend to exist in saturated lipids due to the capability of these radicals to abstract the allylic hydrogen in unsaturated lipids.
Peroxyl and alkoxyl radicals have been identified during oxidation of methyl linoleate employing methyl-N-duryl nitrone (MDN) as the spin trap and observed by EPR (Yamada et al.1984). This technique was also applied for determining the type of structure of metal-dioxygen complexes (Boca1983). This dioxygen coordination can be classified by the formal oxidation state such as superoxo-like (O2 -) or peroxo-like (O2 2-) complexes (Boca1983).
Dikalov et al. investigated the spin trapping of the linoleic and linolenic acid-derived radicals in a mixture of soybean lipoxygenase and 5,5-dimethyl-1-pyrroline N-oxide (DMPO) as the spin trap. Four different radical adducts have been detected in the EPR spectra of radicals derived from linoleic acid as shown in Figure 14 (Dikalov & Mason2001), while EPR spectra of alkoxyl radicals derived from linolenic acid are illustrated in Figure 15.
Despite its ability to analyse radicals arising in the oxidation of linseed oil, the technique exhibits limitations. The detection of free radicals requires low temperature, limiting the use of the instrument to specialised laboratories. The instability of radicals also causes difficulties in achieving an accurate and repeatable analysis, in spite of development of several types of spin traps.
Effect of catalysts on oil oxidation
The types of inorganic pigments added to the paint determine the rate of autoxidation, since the pigments contain transition metals such as cobalt, iron and manganese (Ploeger et al.2009b). Several metal complexes have been reported to catalyse the oxidative drying of alkyd paints, especially metal soaps which significantly accelerate the paint drying (van Gorkum & Bouwman2005). Typical metal soaps, whose role is to accelerate oxidation of paint films and subsequent polymerisation (i.e., drying), correspond to transition metal salts (typically Co, Fe, Mn) of long chain fatty acids (Miccichè et al.2006) with the overall formula of (Men+)(X-)n; where Men+ is a transition metal ion, X- is a C6-C18 aliphatic carboxylate (Miccichè et al.2005a). Metal catalysts have been classified as primary, secondary and auxiliary dryers. Example of primary dryers include Co2+, Mn2+, Ce3+, V3+, Fe2+, secondary Pb2+, Zr4+,Al3+ and auxiliary Ca2+, Li+, K+ and Zn2+ (Meneghetti et al.1998). The primary and secondary dryers become active in the oxidation and polymerisation stages, respectively, while auxiliary dryers modify the activity of primary dryers.
Role of cobalt on oxidation reactions
Cobalt 2-ethylhexanoate dryer has been proven as a particularly effective catalyst to promote the oxidative drying of alkyd paints (Oyman et al.2005b). Cobalt(II) displays better catalytic properties compared to manganese(II) and iron(II), as indicated by higher concentration of products released during oxidation of linseed oil catalysed by cobalt(II) (Juita et al.2011a). Mallégol et al. have shown that the activity of cobalt as primary dryer is modified by the addition of a secondary dryer such as zirconium or a combination of calcium and zirconium (Mallégol et al.2000). The cobalt primary dryer accelerates the hydroperoxide decomposition (Mallégol et al.2000; Tanase et al.2004; Tuman et al.1996), while the zirconium catalyses the further polymerisation (Meneghetti et al.1998; Sharma & Kundu2006); typically Co:Zr ratio has an optimum value of 1:3. It was found that the addition of lead to the cobalt catalyst has no enhancement on the oxidative polymerisation (Meneghetti et al.1998).
According to Sailer et al., cobalt also catalyses the generation of singlet oxygen which is significantly more reactive than ground state oxygen in initiating the formation of the peroxides (Sailer & Soucek2000). Excess of cobalt can lead to the inhibition of the oxidative polymerisation process due to the consumption of peroxyl radicals formed in the reaction (Meneghetti et al.1998). Cobalt enhances the rate of cis-trans isomerisation (De Oliveira Vigier et al.2009). For catalytic mixtures of cobalt and tin, the cis-trans isomerisation decreases, as the tin content increases. This indicates that active sites for this reaction correspond to cobalt species (De Oliveira Vigier et al.2009). The cobalt catalyst accelerates the peroxide formation at the early stage of the oxidation process, as demonstrated by the addition of dicumyl peroxide into the raw rapeseed oil methyl ester (Niczke et al.2007).
Transition metal carboxylates such as cobalt octoate present better catalytic performance compared to manganese or iron (Stava et al.2007). The reactivity of these metals could be improved with some organic ligands such as ferrocene and its derivatives. The catalytic reactivity is improved by altering the electron density at the metal centre of a complex ion, therefore enhancing the redox potential of the metal (Stava et al.2007).
Role of other metals on oxidation reactions
Cobalt has been reported as carcinogenic (Erich et al.2006a; Erich et al.2006b; Liu et al.2007) and genotoxic (Stava et al.2007), prompting a trend to replace cobalt by manganese or iron. Wu et al. reported that the addition of chelating ligands such as 2-aminomethylpyridine and 2-hydroxymethylpyridine can improve the inferior catalytic activity of manganese 2-ethylhexanoate (Wu et al.2004). A Schiff base ligand, which forms in the reaction between 2-pyridinealdehyde and 2-aminopyridine in combination with the manganese salt, was found to be an efficient catalyst for autoxidation of ethyl linoleate, a model compound for linseed oil.
Bipyridine displays excellent catalytic activity in autoxidation if added to manganese 2-ethylhexanoate, but it has a retarding effect on the catalytic activity of cobalt dryers (Warzeska et al.2002). Polyamines, such as 1,1,4,7,10,10-hexamethyl triethylenetetramine (HMTETA) in a complex with manganese, accelerate the oxidation of ethyl linoleate (Oyman et al.2004). Likewise, another polyamine 1,4,7-trimethyl-1,4,7-triazacyclononane (MeTACN) forms a complex with manganese(IV) (MnMeTACN) that catalyses the oxidation of ethyl linoleate (Oyman et al.2004). Working with ethyl linoleate as a surrogate for linseed oil, van Gorkum has reported [Mn(III)(tbpppy)(dpm)] (where H2tbpppy is 2-[bis(2-hydroxy-3,5-di-tert-butylbenzyl)aminomethyl]pyridine and Hdpm is dipivaloylmethane) to be the best potential alkyd paint dryer, acting through a reduction of Mn(III) to Mn(II) (van Gorkum et al.2007). Cobalt and copper salts have been reported to enhance the catalytic activity of the manganese salt in the decomposition of hydroperoxides (Minisci et al.2003).
Iron has not been widely used in the coatings or paints due to observation that iron becomes catalytically active at temperatures above 130°C and its application darkens the paint colour (Miccichè et al.2005a). However, the combination of iron salts with reducing agent, such as ascorbic acid (Miccichè et al.2005b), and nitrogen donor ligands, such as 2-ethyl-4-methylimidazole, has been identified to form an excellent dryer (Miccichè et al.2005a). Combination of ascorbic acid 6-palmitate (AsA6p) and iron reaches the maximum activity at a ratio of three moles of AsA6p to one mole of iron. Fatty acid chain length of 8 to 12 in the ascorbic derivatives is required to provide better catalytic properties (Miccichè et al.2005a) However, Micciche et al. (Miccichè et al.2006) reported that the combination of AsA6p and Fe-2-ethylhexanoate (Fe-eh) achieves the optimum activity towards the oxidation of ethyl linoleate at the molar ratio of two. The addition of monodentate two-nitrogen donor ligands to the AsA6p/Fe improves the drying time and film hardness (Miccichè et al.2005a). Ferrocene derivatives such as 1,1’-dicarbomethoxyferrocene show a synergic effect with cobalt dryer during the oxidation of ethyl linoleate (Stava et al.2007). Soluble iron has been reported to be a much better catalyst compared to iron wire or powder (Colclough1987).
Ioakimoglou et al. have studied the effect of several copper compounds on the oxidation of films of linseed oil. Copper acetate and copper abietate have been found to catalyse the oxidative degradation of linseed oil more efficiently than copper carbonate. However, copper salts with high oxidising capacity possibly inhibit the cross linking reaction (Ioakimoglou et al.1999). Finally, the presence of metal in pigments in the paints may enhance the thermal discolouration and degradation processes of paintings (Ioakimoglou et al.1999).
Copper salts are known as excellent catalysts for the decomposition of peroxides (Kochi & Mains1964), including the decomposition of lipid peroxides (Halliwell & Gutteridge2008). The rate of the decomposition reaction by copper(I) exceeds that by copper(II) (Halliwell & Gutteridge2008). On the other hand, copper(II) represents a better initiation catalyst for radical chains compared to copper(I) (Allen & Patrick1974). Acetyl-acetonate complexes of copper(II) react with hydroperoxides to produce alkyl peroxyl radicals which are effective initiators for polymerisation reactions (Allen & Patrick1974). Copper salt has been reported to accelerate the decomposition of cumene hydroperoxide (Ioakimoglou et al.1999). Transition metals can activate the carbon-hydrogen bond, hence in some cases can cause scission reactions (Vastine & Hall2009).
Inhibitors for oxidation of oils
Certain types of sulfur compounds and aromatics can inhibit the oxidation of mineral oils by radicals, especially peroxyl radicals. For example, this effect is displayed by zinc dialkyldithiocarbamate (ZDC) (Colclough1987). Flavonoids and tocopherol demonstrate antioxidant effects on the lipid oxidation, since the phenolic hydrogens in those substances can react with lipid free radicals (Belhaj et al.2010). Vitamin E is another example, as it is composed of tocopherol. Tocopherol induces several chain breaking reactions, including the reduction of peroxyl and alkoxyl radicals, reactions with carbon centred radicals and the addition of a tocopheroxyl itself to a peroxyl radical (Antunes et al.1996).
Kinetics and reaction mechanisms of oil oxidation
Reaction pathways involved in oxidation
Mechanism of radical chain reactions
The essential processes in the oxidation, involving radical chain reactions, consist of primary initiation, where radicals are formed from the parent molecules, propagation, which conserves the number of radicals, termination that removes the radicals, branching, which multiplies the number of radicals and secondary initiation that forms new radicals from a stable intermediate product (Pilling1997). The rate of oxidation is mostly influenced by the rates of branching and termination.
Antunes et al. (Antunes et al.1996) reported that the primary initiation agents in the basic scheme of lipid peroxidation consist of hydroxyl, perhydroxyl and α-tocopheroxyl radicals. Reactions (7) to (9) describe the abstraction reactions by those radicals. Hydroxyl radical reacts nonselectively with organic compounds (Antunes et al.1996), while the less reactive peroxyl radical abstracts hydrogen selectively at the bis-allylic methylene bridge (Bors et al.1987). Pentadienyl radical, produced by this hydrogen abstraction, has an unpaired electron distributed over five carbon atoms (Bors et al.1987; Gardner1989).
Since the addition of ground state O2 (i.e., triplet oxygen) to biomolecules is spin forbidden, the direct reaction proceeds very slowly with the rate constant of less than 10-5 M-1 s-1 (Miller et al.1990). Thus the critical step in the oxidation of biomolecules, in the presence of atmospheric oxygen, corresponds to the formation of the initial radicals.
The initiation reaction of the oil oxidation can proceed through the formation of singlet oxygen by photosensitised oxidation (Choe & Min2006). Singlet oxygen could be generated from triplet oxygen in chlorophyll photosensitisation, inducing the formation of 2-pentyl furan, trans-2-heptenal and 1-octen-3-ol in linoleic acid samples (Lee & Min2010). Chlorophyll, commonly found in vegetable oils, serves as a photosensitiser. Photosensitisers absorb light energy very rapidly and convert the singlet state to excited triplet state sensitisers. The reaction of these excited sensitisers with triplet oxygen produces singlet oxygen (Choe & Min2006; Min & Boff2002; Rawls & Santen1970). The reactions involving singlet oxygen are probably responsible for initiating the self-heating of linseed oil. While autoxidation incorporates the formation of alkyl radicals, photosensitised oxidation involves the reaction between singlet oxygen and double bonds without the formation of these alkyl radicals to generate hydroperoxides at the double bonds (Choe & Min2006).
Reactions with singlet oxygen exhibit low activation energies, 0 to 25 kJ mol-1, allowing facile oxidation of biomolecules (Lee & Min2010). Different hydroperoxides arise from reactions involving singlet and triplet oxidation. The former generates C9, C10, C,12 and C13 hydroperoxides and the later, known as autoxidation, forms C9 and C13 hydroperoxides (Lee & Min2010).
Propagation reactions proceed through four dominant pathways: atom transfer, electron transfer, addition and scission reactions. Abstraction of hydrogen represents the common reaction of atom transfer in the propagation step (Roberfroid & Calderon1995), for example the abstraction of hydrogen from fatty acid chain by peroxyl radical (Halliwell & Gutteridge2008). The combination of molecular oxygen with an electron to form superoxide characterises the second type of propagation via electron transfer (Roberfroid & Calderon1995). In this reaction, an electron transfers from a transition metal ion to a peroxide compound. The third pathway operates in the lipid peroxidation and involves the addition of oxygen to the alkyl radical (Roberfroid & Calderon1995). This reversible reaction of oxygen with an initial carbon centred radical to form a peroxyl radical constitutes the most important pathway (Antunes et al.1996). It is controlled by oxygen migration with the apparent activation energy of 24 kJ mol-1 in unsaturated lipids (Zhu & Sevilla1989). Kinetically, it is a very fast pathway, hence the concentration of peroxyl radicals is much higher than alkyl radicals (Hanson et al.2004; Kubow1992). The last of the four dominant propagation pathways involves the transformation of peroxyl radicals into allylic and pentadienyl radicals (Zhu & Sevilla1989).
Peroxyl radicals can abstract hydrogen to form hydroperoxides. Hydrogen abstraction by radicals is least favourable from alkanes, more favourable from a monoallylic position in alkenes and most favourable from a double allylic position, as a consequence of decreasing C-H bond dissociation energies (Oyman et al.2005a). In addition to the hydrogen abstraction from substrate, peroxyl radicals also play an important role in the abstraction of the weakly bonded α hydrogen atom of the hydroperoxide product (Figure 16) as described in reactions (11) and (12). The decomposition of R-αH •OOH (αH refers to the weakly bonded α hydrogen) to Q = O (Q denotes R-αH) and •OH releases around 126 kJ mol-1. The hydroxyl radical rapidly abstracts a hydrogen atom from substrate and this reaction is also exothermic (Hermans et al.2007).
Moreover, peroxyl radical could undergo cyclisation reactions by the intramolecular arrangement through four and five membered rings. Quantum chemical calculations identified low energy pathways for the decomposition of cyclic peroxides into aldehydes and ketonic species (Juita et al.2011e).
An alkoxyl radical forms as the result of the decomposition of lipid hydroperoxide by transition metals such as Fe2+. These radicals can abstract hydrogen from unsaturated fatty acid or add to double bonds to form carbon centred radicals (Antunes et al.1996). Heteroatom-centred radicals display higher ratios of hydrogen abstraction to addition than carbon-centred radicals. For instance, an alkoxyl radical prefers to abstract hydrogen over addition due to the difference in exothermicity between the two reactions of 30 kJ mol-1 (Moad & Solomon1995).
The termination reactions proceed through the radical-radical recombination or cross linking reaction. The reaction between two peroxyl radicals predominates over reaction between carbon-centred radicals and between a carbon-centred radical and a peroxyl radical (Antunes et al.1996). Recombination of the carbon-centred radicals has an apparent activation energy of 40 kJ mol-1 (Zhu & Sevilla1989). The rate constant for the termination reaction depends on the chain lengths of the two radicals and it is affected by the diffusion mechanisms (Moad & Solomon1995). The primary radicals involved in the polymerisation reaction are hydroxyl and alkoxyl radicals (Allen & Patrick1974). Cobalt(II) complexes, as the chain transfer agents, can catalyse the polymerisation reaction through the combination of metal with carbon-centred radicals (Moad & Solomon1995).
The addition of oxygen to a free radical and the termination step by crosslinking involve exothermic processes, while decomposition of hydroperoxides constitutes an endothermic process. The addition of dryers and metal alkoxides can decrease the onset temperature of the reaction exotherm. This affects the extent of curing, as measured by the indentation hardness of the drying films (Tuman et al.1996).
In the autoxidation reaction of cyclohexane (CyH), which has similarity with that of linseed oil, the major chain initiation reaction is the homolytic dissociation of CyOOH into CyO• and •OH radicals, while the major termination step is the reaction of two peroxyl radicals into CyOH, Q = O and O2. The hydrogen abstraction reaction by peroxyl radical represents the rate determining step in the chain propagation reaction. The addition of oxygen to the cyclohexane radical is diffusion controlled under sufficient oxygen pressure (Hermans et al.2006).
Isomerisation reactions and hydroperoxides as important intermediates
Thermal decomposition of peroxides proceeds mostly through the homolytic fission of the O-O bond (Swern1972), as a consequence of the low dissociation energy of this bond. Cobaltous ion assists the decomposition of hydroperoxide into alkoxyl radical (Yamada et al.1984). The rate of hydroperoxide decomposition increases at elevated pH (Rolewski et al.2009). Hydroperoxides formed during oxidation of lipid represent stable intermediates, therefore, their detection indicates the initial lipid oxidation (Rolewski et al.2009). The amount of monohydroperoxides decreases with increasing temperature due to the faster degradation of monohydroperoxides (Haslbeck et al.1983). Linseed oil produces hydroperoxide at abundances one order of magnitude higher than linoleic acid due to a large modal content of linolenic acid in linseed oil (Rolewski et al.2009).
Table 7 illustrates different hydroperoxide species that form from oxidation of methyl esters of oleic, linoleic and linolenic acids. For each methyl ester, the product species differ by the position of double bonds and the location of OOH attachment. Oxidation of linoleic esters produces almost exclusively 9- and 13-hydroperoxides, which are categorised as outer hydroperoxides. Inner hydroperoxides such as 10- and 12-hydroperoxides arise in minor amounts, possibly as a consequence of other reaction pathways, leading to formation of cyclic peroxides (i.e., endoperoxides) and resulting in smaller amounts of inner hydroperoxides, as illustrated in the following scheme (Gunstone1996; Roberfroid & Calderon1995; Halliwell & Gutteridge2008) Scheme 4.
This cyclic peroxide forms when a peroxyl radical attacks another double bond in the same compound (Halliwell & Gutteridge2008).
Oxygen concentration influences the mechanism of peroxidation. For instance, self reaction of carbon centred radicals is more likely to occur at a very low concentration of oxygen (Halliwell & Gutteridge2008). As a consequence of this sensitivity, many complex mixtures of compounds can be produced from the decomposition of lipid peroxides, involving epoxides, saturated and unsaturated aldehydes, ketones and hydrocarbons (Halliwell & Gutteridge2008).
There are literature reports on the mechanistic differences between the oxidation of conjugated and non-conjugated fatty acids. The H abstraction rate of non-conjugated fatty acid is faster than that of the conjugated acids. This is because of resonance stabilisation of conjugated double bonds and also the presence of trans double bonds (Muizebelt et al.2000). For example, conjugated ethyl linoleate is estimated to be 12–17 kJ mol-1 more stable than non-conjugated ethyl linoleate (Muizebelt et al.2000). For comparison, radicals arising from H abstraction from non-conjugated fatty acids are stabilised by resonance. This results in lower abundance of peroxide species present in oils containing conjugated fatty acids (Oyman et al.2005a). Polymerisation reactions can occur by direct addition of free radicals to the conjugated double bonds and by radical recombination (Oyman et al.2005a). Conjugated fatty acids tend to favour radical addition to double bonds, whereas non-conjugated species prefer to enter into radical recombination reactions (Muizebelt et al.2000).
A cis, non-conjugated diene structure changes to a cis,trans conjugated hydroperoxide during autoxidation of methyl linoleate or methyl linolenate (Hendriks et al.1979). A similar transformation also takes place during hydrogenation of unsaturated lipids. As for the autoxidation reactions, the ratio of cis to trans as the result of the partial hydrogenation of oil depends on the initial oil composition, catalyst and temperature (De Oliveira Vigier et al.2009).
The isomerisation reaction of hydroperoxides during oxidation of linseed oil is similar to the mechanism that occurs in isoprene oxidation. Peeters et al. studied the addition of hydroxyl radical to isoprene and found the major adducts to be 1-OH (about 60%) and 4-OH (around 30%). Trans isoprene is the more stable and dominant structure which releases 159 kJ mol-1 of energy upon OH addition to form around 50% trans- and 50% cis-1-OH. The reaction entails the formation of an allyl resonance configuration permitting addition at two positions. These activated adducts can undergo cis-trans isomerisation with the barriers of only 59–63 kJ mol-1. The addition of oxygen to the OH-isoprene occurs rapidly at two addition sites, as illustrated in the third row of Figure 17. The substitution at a β site enables the internal rotation of the –CH = CH2 group, forming both cis and trans OH-isoprene radicals. Addition to the α sites induces the formation of E- and Z-substituted alkenes. β-OH-peroxyl radicals undergo the 1,5-H shift of the OH-hydrogen to form a β-HOO-alkoxyl radical, which displays a very low barrier to further decomposition. Additionally, the Z-1-OH-4-OO• can isomerise via 1,6-H-shift of the weakly bonded α-OH hydrogen to the peroxyl radical site by overcoming a barrier of 75 kJ mol-1 (Peeters et al.2009; Stavrakou et al.2010).
Mechanism of epoxidation reactions
The epoxidation of alkene has been proposed to occur by a butterfly mechanism that involves the peroxyl oxygen atom of the peracid attaching to a double bond and the hydrogen atom of the peracid connecting with the carbonyl oxygen (Okovytyy et al.2002). Hilker et al. described the chemo-enzymatic epoxidation of unsaturated plant oils in which the peracid transfers an oxygen atom to the double bond to form epoxide. This is called the Prilezhaev reaction, as described in Figure 18 (Hilker et al.2001). This reaction has been reported to be second order, first order in perstearic acid and in double bonds, with an activation energy of 51.2 kJ mol-1 (Hilker et al.2001).
Oxygen in an epoxide ring can be attacked by acid causing the opening of the ring and the formation of a hydroxyl species and a carbocation (Chiniwalla et al.2003). Crosslinking can occur when the oxygen of an epoxide group is attacked by a carbocation (Chiniwalla et al.2003). A naturally epoxidised vegetable oil, vernonia oil, employed in formulations of alkyd and epoxy coating, consists of a triacylglycerol of vernolic (cis-12,13-epoxy-cis-9-octadecenoic) acid. It contains one epoxy ring and one double bond separated by a single methylene group in each acid chain (Muturi et al.1994. The epoxidation process increases the viscosity of the oil. The fully epoxidised oil possesses no double bonds therefore it cannot dry further (Muturi et al.1994). Oligomeric cobalt complexes have been utilised as catalysts for hydrolysis of epoxides compounds, causing the opening of the epoxide ring to form hydroxyl compounds (Ready & Jacobsen2002).
Catalytic pathways involving transition metals
Four modes of catalytic reactivity have been reported to operate in the presence of transition metal complexes, including: (1) free radical autoxidation; (2) coordination of an organic substrate to a metal centre which is followed by reaction of an oxygen-containing nucleophile; (3) oxygen atom transfer from a high valent oxo-metal compound; and, (4) decomposition of hydroperoxide catalysed by metal (Bailey & Drago1987). Organometallic cobalt derivatives enhance the polymerisation reactions of olefins through the coordinative and radical pathways, illustrated in Figure 19 (Milani et al.2003). The coordinative pathway occurs through the dissociation of the Co-N (cobalt-nitrogen) bonds resulting in the formation of a unsaturated coordination complex, while radical polymerisation reaction is initiated by the homolytic cleavage of the cobalt-carbon bond (Milani et al.2003).
Catalytic mechanism of decomposition of hydroperoxides
Transition metal complexes have been reported to catalyse the decomposition of hydroperoxides into peroxyl radical or alkoxyl radical (Roberfroid & Calderon1995; Aust et al.1985). The reaction of metal in a lower oxidation state with peroxides is generally faster than that involving metal in a higher oxidation state (Kochi1973). Iron(II) chelates can split the O-O bond in the peroxide to produce an alkoxyl radical. On the other hand, a peroxyl radical is formed in the presence of iron(III) (Halliwell & Gutteridge2008). Iron(II) induces a higher rate of hydroperoxide decomposition than iron(III). However, too high an amount of iron(II) can scavenge radicals hence the ratio of iron(II) to peroxides affects the kinetic pathways of peroxidation reactions (Halliwell & Gutteridge2008).
During oxidation of benzoin (2-hydroxy-1,2-di(phenyl)ethanone or PhCH(OH)C(O)Ph) catalysed by Co(II)(acac)2, oxygen reacts with Co(II)-benzoin complex to form Co(III)2(O2 2-)(PhCHO-COPh), therefore cobalt(II) converts to cobalt(III). Cobalt(II) nitrate has been reported as the most active catalyst, among other cobalt(II) salts, for the oxidation of benzoin in acetonitrile solvent due to stronger interaction between cobalt(II) nitrate and acetonitrile molecule compared to the interaction involving cobalt(II) halide (Tsuruya et al.1981).
As already well recognised in this review, oxidation of linseed oil involves peroxyl chemistry, displaying importance to other industrial applications, such as that described by Turra et al. (Turra et al.2010). These authors reported the mechanism of the decomposition of tert-butylhydroperoxides catalysed by cobalt(II) acetylacetonate (in cyclohexane). The first step involves the reaction of cobalt(II) and hydroperoxide to form alkoxyl radical and CoIII-OH compound with the activation energy of 54 kJ mol-1. This species then reacts with another hydroperoxide to regenerate cobalt(II) and produce peroxyl radical in the second step which is part of the Fenton-like cycle. Alcohols display a tendency to inhibit catalytic deperoxidation reactions by cobalt which probably occurs due to the coordination of alcohol to cobalt species. Quantum calculation predicted that reaction (17) remains slightly slower than reaction (18). The rate of this deperoxidation decreases at higher cobalt concentration, for instance at 333 K, the rate decreases once the concentration of Co(acac)2 exceeds 100 μM (Turra et al.2011). The proposed explanation invokes the termination reaction of the Co III-OH to form a stable bis(μ-hydroxo) dimer, Co-(OH)2-Co,(Turra et al.2010) as illustrated in the following Scheme 5:
Reaction (22) controls the propagation rate of the overall mechanism of the decomposition of hydroperoxide.
Alkoxyl radicals arise as the main products of the self reaction of tertiary peroxyl radical reactions (reaction 22), while the minority pathway comprises the termination reaction (23), with about 10% of the ROO radicals consumed through reaction (24) (Turra et al.2010). Alkoxyl radical produced by reaction (17) can interact with the cyclohexane (CyH) solvent (20) or with the hydroperoxide (21), however, reaction (21) consumes the majority of the alkoxyl radicals (Turra et al.2010).
The presence of a metal catalyst decreases the activation energy required to decompose two moles of hydroperoxide from 90–170 to 40–50 kJ mol-1 via reactions (25) and (26) (Mallégol et al.2000). The activation energy required for the reaction between oxygen and unsaturated fatty acid esters in the presence of cobalt dryers is only one tenth of that in the absence of cobalt dryer (Skalský1976). The activation energies for the decomposition of hydroperoxyl linoleic and linolenic acid methyl ester at 25°C are 36 kJ mol-1 and 38.5 kJ mol-1, respectively (Hendriks et al.1979). In contrast, cobalt complexes may stabilise the resulting peroxidic product through the coordination (Nishinaga et al.1988). In practice, peroxide value serves to estimate the extent of oxidation and curing.
Catalytic mechanism of complex formation
Cobalt(II)-Schiff base complexes can react with oxygen to form dioxygen complexes (Nishinaga et al.1988); Nishinaga et al. reported that cobalt(II) complex displays a four-coordinate structure (Nishinaga et al.1988). This reaction appears non-radical in nature, and entails the formation of a charge transfer complex, [CoIII(O2 -)], in an energetically favourable transformation (Boca1981). In this process, an electron transfers from Co(II) to the coordinated dioxygen to form superoxide, Co(III)(O2 -) (Boca1981). In comparison, a general reaction of the first row transition metals represents the radical chain autoxidation (Bailey & Drago1987; Nishinaga et al.1988). The activation of dioxygen through coordination to cobalt(II) complexes produces a strong oxidant (Boca1983). The initiation step in the oxidation of substrate proceeds as follows (Boca1981):
Dioxygen complexes are well known for their role in biological processes and in catalysis (Boca1983). The dioxygen activation is indicated by the O-O bond lengthening and Co-O distance shortening (Boca1983). Transition metal complexes fulfil important roles in activating the molecular oxygen through the coordination and partial reduction of oxygen (Bakac2010). Smeets et al. investigated the coordination and activation of oxygen employing transition metal ions in zeolites (Smeets et al.2010). The metal and oxygen oxidation states undergo changes during the activation process. Ligands coordinating to the metals (Fukuzumi et al.2002) control the redox reactivities of transition metal ions. Most organic radicals can reduce oxygen to form superoxide due to their redox potentials (Roberfroid & Calderon1995).
Kinetic parameters for radical chain reactions
The relative stabilities of peroxyl radicals in triacylglycerols follow the order from the most stable tristearin, to triolein, trilinolein and trilinolenin. There is no formation of peroxyl radicals below 100 K, the peroxidation starts at 105 K. The activation energies for the decomposition of peroxyl radicals into allylic and pentadienyl radicals in triolein, trilinolein and trilinolenin amount to 88 ± 11, 34 ± 8 and 9 ± 2 kJ mol-1, respectively (Zhu & Sevilla1989).
The autoxidation reaction of methyl linoleate and methyl linolenate have been reported to be first order in the ester concentration at temperatures between 25 and 75°C (Hendriks et al.1979). The ratio of rate constants for oxidation of mono, di and triunsaturated esters have been obtained at 90°C as 1:3:12 (Litwinienko & Kasprzycka-Guttman1999). The activation energies of oxidation of methyl oleate, linoleate and linolenate derived from DSC measurements correspond to 95.0 ± 4.7, 76.4 ± 5.0 and 74.5 ± 8.2 kJ mol-1, respectively (Litwinienko & Kasprzycka-Guttman1999). The addition of dryers reduces the activation energy for the onset of autoxidation of linolenic acid from 71 kJ mol-1 by about two thirds (Howitt et al.1995). The initial oxidation temperature of unsaturated fatty acids is lower by 20°C compared to that of esters of the same fatty acids (Litwinienko & Kasprzycka-Guttman1999).
Lipid peroxidation in biological systems has been modelled for reactions involving polyunsaturated fatty acids (PUFA), tissue activator enzymes, antioxidants and glutathione, with lipid peroxidation representing the main pathways (Tappel et al.1989). Rate constants for several important reactions in the lipid peroxidation are listed below (Antunes et al.1996):1.
L stands for lipid, P refers to phospholipid and Toc denotes tocopherol.
Halliwell and Gutteridge (Halliwell & Gutteridge2008) reported the rate constant of the reaction of carbon-centred radicals with oxygen to form peroxyl radicals to be greater than 109 M-1 s-1. Peroxyl and alkoxyl radicals, known as good oxidising agents, play important roles in lipid peroxidation, especially as agents for hydrogen abstraction from the lipid molecules (Halliwell & Gutteridge2008). Table 8 lists the reaction rate constant of hydrogen abstraction from unsaturated fatty acid by several kinds of radicals and that of oxygen reaction with alkyl radical.
The oxidative cross linking process of linseed oil in the presence of metal catalysts is not autocatalytic at temperatures between 120 to 155°C (Tuman et al.1996). Non-conjugated trienoic acid (fatty acid) esters undergo thermal polymerisation reaction at 275°C with much lower rate compared to the various conjugated trienoic esters (Ault et al.1942). Furthermore, the rate of oxidative polymerisation is lower than the autoxidation process (Litwinienko & Kasprzycka-Guttman1999).
Güler et al. (Güler et al.2004) studied the kinetics of oxypolymerisation reaction of linseed oil by estimating the rates of reactions from changes in viscosity and found that the reaction order varied with temperature. At 200, 120 and 80°C and for the flow rate of air of 2 dm3 min-1, the reactions were first, second and third order, respectively, indicating a complex elementary reaction mechanism underlying the observed overall kinetics. The drying performance of oil with a high percentage of linolenic acid is faster and the induction time is shorter compared to an oil rich in linoleic acid. This is because oil rich in linolenic acid tends to form a skin layer, causing a diffusion barrier to oxygen resulting in a high level of residual unsaturation in the film (Stenberg et al.2005).
Another practical study examining the overall kinetics was performed by Khattab et al. (Khattab et al.1999) who investigated the effect of contamination of cotton fabric with linseed oil on the activation energies of pyrolysis and oxidation of the fabric. These authors described the apparent activation energy of the gaseous oxidation product (E ox) by the following expression:
where E p is the apparent activation energy of pyrolysis, T i denotes the onset of spontaneous ignition, H r corresponds to heating rate, and A p stands for the apparent Arrhenius factor describing the pyrolysis. The presence of oil in fabrics reduces the activation energy of pyrolysis and oxidation, and increases oxygen demand, demonstrating the sensitivity of the process to oxygen concentration (Khattab et al.1999).
Digression on cool flames
Peroxide chemistry is also important in low temperature combustion, in the temperature window associated with cool flames, followed by a region denoted as the negative temperature coefficient (where the oxidation rate decreases with increasing temperature), and then by the fuel auto-ignition. A significant body of literature exists on these subjects, as recently reviewed by Battin-Leclerc (Battin-Leclerc2008) and prior to that by Pilling (Pilling1997). In particular, these reviews have illustrated the important role of thermodynamics on peroxide reactions, at increasing temperatures. The effect of temperature on the peroxidation reaction of alkyl radical is described below by equations 36 to 38. The rate of oxidation increases significantly as temperature rises in the window of 500 to 600 K, since the equilibrium moves to the right. On the other hand, at higher temperature of above 600 K the oxidation rate falls as the equilibrium shifts to the left, causing the amount of hydroperoxides formed to decrease.
Linseed oil has found numerous applications in painting, varnishes, wood treatment and linoleum due to its drying properties. However, in the presence of a metal catalyst, the oxidation of linseed oil soaked into lignocellulosic materials, such as cotton rags, may induce their spontaneous heating. As a consequence of the importance of the unsaturated fatty acid (present in linseed oil as triacylglycerols) to nutrition, several studies have been undertaken to investigate the oxidation of the active components in linseed oil; often deploying surrogate compounds, such as ethyl linoleate. These studies led to establishing a generally good understanding of the overall radical chain reactions that operate in the oxidation process. The chemical reactions involve initiation, propagation and termination steps. They comprise oxygen addition to the radicals, formation of hydroperoxides, which are known to be important intermediates, and decomposition of peroxides leading to the formation of volatile organic species and higher molecular weight compounds. However, critical details have eluded comprehension, such as the emergence of the initial radicals, the detailed mechanistic understanding of important chemical species observed in experiments, or the role of most of the transition metals suspected to induce catalytic enhancement of the oxidation reactions.
Several experimental methods have been developed to evaluate the oxidation and self-heating tendency of linseed oil and cotton. Likewise, numerous analytical methods have been applied to identify and quantitate the chemical changes during oxidation, the gaseous and liquid products and the catalytic effect of metal dryers. The chemical structure of the triacylglycerols in the oil determines the reactivity of the species in the radical chain reactions. The significant catalytic pathways proceed either through the decomposition of hydroperoxides or formation of coordination complexes. However, only a limited number of kinetic parameters has been reported for catalytic reactions.
The pathways that operate in the oxidation of linseed oil include those which are known to occur in other applications. For instance, biological systems involve radical chain reactions of lipid peroxidation, and polymer systems comprise crosslinking reactions that rely on the recombination of radicals. Peroxides are formed in low-temperature oxidation of hydrocarbons, and the thermal runaway reactions, involving peroxides, are similar to those that occur in self-heating and autoignition of linseed oil.
The classical models of ignition oversimplify the complexity of the peroxyl chemistry involved in the oxidation reactions. Knowledge of detailed reaction chemistry is required to evaluate the propensity of a material to self-heating from its chemical composition. Although the pathways of the autoxidation and polymerisation have been investigated, detailed mechanisms have not yet been proposed to explain the formation of products identified in experiments. A limited number of publications in literature discusses the formation of carbon dioxide and carbon monoxide during oxidation reactions of linseed oil. Because of its practical importance in the paint industry, previous researchers have mainly focussed on gaining insights into the oxidative drying of oils. Very little effort has gone into unravelling the chemical reactions that govern the self-heating of linseed oil, and considerable research still needs to be conducted to obtain a better understanding of the chemistry of this phenomenon.
This review has demonstrated that the initiation reactions involve singlet oxygen that forms in the presence of light and organic species, such as chlorophyll. The propagation reactions involve triplet (i.e., ground state) oxygen. The present models of self-ignition do not account for this behaviour, and their predictions need to be viewed with great scepticism. For example, the study of Worden, performed with boiled linseed oil, could not even identify subcritical conditions. A significant range of kinetic parameters exists in the reported values, as a consequence of different types of linseed oil being tested and experimental methodologies. This situation is hardly acceptable from the perspective of fire safety as at present no reliable predictions can be made of ignition behaviour of lignocellulosic materials soaked with linseed oil. Further experimental and theoretical progress in the field is urgently needed.
Appendix A. Kinetic parameters from Chen, Jones and adiabatic methods
A.1. Comparison of Chen and Jones’ methods
Both methods assume constant and isotropic material properties, and no water evaporation. The oxygen diffusion in the sample is taken to be fast in comparison to oxidation (i.e., Damköhler number is small) and that oxidation itself is not limited by the depletion of the fuel. This allows decoupling of the mass conservation equations (for fuel and oxygen) from the heat conduction equation simplifying the problems to the solution of the latter. The validity of this assumption has not yet been tested for lignocellulosic materials soaked with linseed oil.
Both methods also assume that the overall oxidation reaction is first order with respect to the fuel; i.e., the effect of oxygen concentration is incorporated in the pre-exponential factor A. This assumption is much more severe. As demonstrated in this review, the oxidation mechanism constitutes a complex set of radical reactions, which, in the case of boiled oil, are accelerated by a metal catalyst. The initiation reactions involve singlet oxygen and the propagation reactions entail triplet oxygen. From this perspective, the kinetic parameters (A and E) obtained by either Jones’ or Chen’s method can only be considered as global in nature; i.e., they represent the effect of the entire chemical mechanism condensed into one global chemical reaction.
As will be demonstrated below, Chen’s method introduces no additional assumption, which makes the estimates of A and E to correspond to global but intrinsic material properties. That is, Chen’s method yields kinetic constants of fundamental importance. On the other hand, Jones’ method comprises an additional assumption that the thermal behaviour of a sample can be described by an average temperature, as measured at the sample’s centre. This results in the estimates of A and E to be apparent. Such estimates can be applied to rank various materials (e.g., coals) for their propensity to spontaneous ignition. However, these estimates convey no fundamental meaning.
A.2. Derivation of the heat balance equation underlying Chen’s method
The derivation of Chen’s method starts with the consideration of a differential element near the sample’s centre. Imagine that one thermocouple is placed at the centre (T C) and one to the side (T S). As the time progresses, the temperature profile in the sample will evolve is illustrated below. The locations of the thermocouples are illustrated above in (Scheme 6).
At certain instant, T C and T S will cross each other. Now, let us imagine a basket with a differential (i.e., very small) element in its centre as shown in (Scheme 7).
For simplicity, in further considerations, we assume a one dimensional case. For this reason, the volume of the differential element is simply Δx × 1 × 1 (Scheme 8).
At crossing point, T C = T S, the heat fluxes through P W and P E disappear, since
where k W and k E denote the heat conduction coefficient (W m-1 K-1). Then, the differential energy balance yields
where, A is pre-exponential factor (s-1), Q enthalpy of the oxidation reaction (J kg-1), c heat capacity of the bulk sample (J kg-1 K-1), and ρ bulk density of the sample (kg m-3). Taking the natural logarithm yields the celebrated Chen equation
Performing experiments for several T C, one plots versus to obtain estimates of intrinsic E and A (or QA), in a least square sense.
A.3. Derivation of the heat balance equation underlying Jones’ method
The derivation of Jones’ method starts with an integral heat balance around the basket, with the control surface corresponding to the gauze surface. The material in the basket is assumed to be at an average temperature T 1 aver, as measured at the centre of the sample (Scheme 9). The heat transfer across the control surface proceeds by convection. This leads to the following time-dependent ordinary differential equation
where a denotes the dimension of the cube (m), T 1 aver average sample temperature (K), as measured by thermocouple 1 at the centre point of the sample, T 2 – oven temperature (K), as measured by thermocouple 2 near the sample surface, and other symbols have their standard meaning. At the crossing point temperature (T 2 = T 1 aver), the above equation leads to
A simple algebraic manipulation then yields the Jones equation
This demonstrates that the kinetic parameters are obtained at an average temperature of the sample. This temperature is taken to correspond to the temperature at the centre of the sample. It is not the true average temperature of the sample. This means that Jones’ method yields apparent kinetic parameters of the sample as opposed to the intrinsic (i.e., fundamental) parameters derived by Chen’s method. Though, do not forget that, both estimates simplify the detailed radical and catalytic chemistry of the oxidation of linseed oil to one global kinetic step.
The apparent values of E and A are obtained by plotting versus, from several experiments, analogously to Chen’s method.
We conclude this Appendix by observing that, the equation to obtain A and E from an adiabatic reactor has the same limitations as that of Jones. In the adiabatic reactor, the integral energy balance also yields
where T C,aver denotes the temperature of the sample, as measured at its centre. However, for an adiabatic reactor, only a single experiment is needed to obtain the apparent values of A and E. Complexity of the adiabatic reactor system could be substantial, especially the control system, to ensure no heat-flux conditions at the walls of the sample.
Allylic Position Equivalent
Ascorbic acid 6-palmitate
Bis-Allylic Position Equivalent
Cyclic Fatty Acid Monomers
Dynamic Headspace-Thermal Desorption
Differential Scanning Calorimetry
Differential Thermal Analysis
Electron Paramagnetic Resonance (same as ESR)
Electrospray Ionisation- Mass Spectrometry
Electrospray Ionisation- Mass Spectrometry/Mass Spectrometry
Electron Spin Resonance (same as EPR)
Fatty Acid Methyl Esters
Fourier Transform Infrared Spectroscopy
Gas Chromatography–Mass Spectrometry
Gas Chromatography- Time-of-Flight Mass Spectrometry
Hydrogen-Nuclear Magnetic Resonance
High Performance Liquid Chromatography
Liquid Chromatography-Mass Spectrometry
Liquid Chromatography-Mass Spectrometry/Mass Spectrometry
Matrix-assisted laser desorption/Ionisation-mass spectrometry
Matrix-Assisted Laser Desorption/Ionisation-Reflectron Time of Flight-Mass Spectrometry
National Fire Protection Association
Olfactory Detector Outlet
Polyunsaturated Fatty Acid
Size Exclusion Chromatography
Secondary Oxidation Products
Solid Phase Microextraction
Solid Phase Microextraction-Gas Chromatography–Mass Spectrometry
Thermal Desorption Gas Chromatography combined with Olfactometry and Mass Spectrometry
Thermogravimetric Analysis- Differential Scanning Calorimetry
Thermogravimetric Analysis- Differential Scanning Calorimetry- Fourier Transform Infrared Spectroscopy
Thermogravimetric Analysis- Differential Scanning Calorimetry- Mass Spectrometry
Thermogravimetric Analysis- Differential Thermal Analysis
Time-of-Flight Mass Spectrometry
Time Resolved-Fourier Transform Infrared Spectroscopy
Ultra High Purity
Volatile Organic Compounds
Zero Point Vibrational Energies
Micro Gas Chromatography.
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This study was supported by a grant from the Australian Research Council. Juita thanks the University of Newcastle for a postgraduate scholarship.
The authors declare that they have no competing interests.
The first two authors shared equally writing and revising the manuscript. EMK assisted with drafting the manuscript. JCM contributed to the section on kinetics and reaction mechanisms of oil oxidation. All authors read and approved the final manuscript.